Abstract
This paper presents the design and microfabrication of a vacuum-based cell holding device for single-cell immobilization and the use of the device in mouse zygote microinjection. The device contains many through-holes, constructed via two-sided glass wet etching and polydimethylsiloxane (PDMS)-glass bonding. Experimental results of mouse zygote immobilization and microinjection demonstrate that the device is effective for rapid cell immobilization and does not produce negative effect on embryonic development.
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Acknowledgements
This work was supported by the Natural Sciences and Engineering Research Council of Canada, the Ontario Ministry of Research and Innovation, and the Ontario Centers of Excellence. We also acknowledge the financial support from the Canada Research Chairs program to YS, and the Ontario Graduate Scholarship program to XL.
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An erratum to this article can be found at http://dx.doi.org/10.1007/s10544-009-9359-8
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Liu, X., Sun, Y. Microfabricated glass devices for rapid single cell immobilization in mouse zygote microinjection . Biomed Microdevices 11, 1169–1174 (2009). https://doi.org/10.1007/s10544-009-9333-5
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DOI: https://doi.org/10.1007/s10544-009-9333-5