Microfabricated glass devices for rapid single cell immobilization in mouse zygote microinjection
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- Liu, X. & Sun, Y. Biomed Microdevices (2009) 11: 1169. doi:10.1007/s10544-009-9333-5
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This paper presents the design and microfabrication of a vacuum-based cell holding device for single-cell immobilization and the use of the device in mouse zygote microinjection. The device contains many through-holes, constructed via two-sided glass wet etching and polydimethylsiloxane (PDMS)-glass bonding. Experimental results of mouse zygote immobilization and microinjection demonstrate that the device is effective for rapid cell immobilization and does not produce negative effect on embryonic development.