, Volume 11, Issue 6, pp 1169-1174
Date: 04 Jul 2009

Microfabricated glass devices for rapid single cell immobilization in mouse zygote microinjection

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Abstract

This paper presents the design and microfabrication of a vacuum-based cell holding device for single-cell immobilization and the use of the device in mouse zygote microinjection. The device contains many through-holes, constructed via two-sided glass wet etching and polydimethylsiloxane (PDMS)-glass bonding. Experimental results of mouse zygote immobilization and microinjection demonstrate that the device is effective for rapid cell immobilization and does not produce negative effect on embryonic development.

An erratum to this article can be found at http://dx.doi.org/10.1007/s10544-009-9359-8