Biomedical Microdevices

, Volume 11, Issue 4, pp 893-901

First online:

Localized surface plasmon resonance biosensor integrated with microfluidic chip

  • Chengjun HuangAffiliated withInteruniversity Microelectronics Center (IMEC)Department of Electrical Engineering (ESAT), Katholieke Universiteit (KU) Leuven Email author 
  • , Kristien BonroyAffiliated withInteruniversity Microelectronics Center (IMEC)
  • , Gunter ReekmansAffiliated withInteruniversity Microelectronics Center (IMEC)
  • , Wim LaureynAffiliated withInteruniversity Microelectronics Center (IMEC)
  • , Katarina VerhaegenAffiliated withInteruniversity Microelectronics Center (IMEC)
  • , Iwijn De VlaminckAffiliated withInteruniversity Microelectronics Center (IMEC)Department of Electrical Engineering (ESAT), Katholieke Universiteit (KU) Leuven
  • , Liesbet LagaeAffiliated withInteruniversity Microelectronics Center (IMEC)
  • , Gustaaf BorghsAffiliated withInteruniversity Microelectronics Center (IMEC)

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A sensitive and low-cost microfluidic integrated biosensor is developed based on the localized surface plasmon resonance (LSPR) properties of gold nanoparticles, which allows label-free monitoring of biomolecular interactions in real-time. A novel quadrant detection scheme is introduced which continuously measures the change of the light transmitted through the nanoparticle-coated sensor surface. Using a green light emitting diode (LED) as a light source in combination with the quadrant detection scheme, a resolution of 10−4 in refractive index units (RIU) is determined. This performance is comparable to conventional LSPR-based biosensors. The biological sensing is demonstrated using an antigen/antibody (biotin/anti-biotin) system with an optimized gold nanoparticle film. The immobilization of biotin on a thiol-based self-assembled monolayer (SAM) and the subsequent affinity binding of anti-biotin are quantitatively detected by the microfluidic integrated biosensor and a detection limit of 270 ng/mL of anti-biotin was achieved. The microfluidic chip is capable of transporting a precise amount of biological samples to the detection areas to achieve highly sensitive and specific biosensing with decreased reaction time and less reagent consumption. The obtained results are compared with those measured by a surface plasmon resonance (SPR)-based Biacore system for the same binding event. This study demonstrates the feasibility of the integration of LSPR-based biosensing with microfluidic technologies, resulting in a low-cost and portable biosensor candidate compared to the larger and more expensive commercial instruments.


Localized surface plasmon resonance (LSPR) Gold nanoparticle Microfluidic Biosensor