Article

Biomedical Microdevices

, Volume 11, Issue 4, pp 783-793

Novel biological/biohybrid prostheses for the ossicular chain: fabrication feasibility and preliminary functional characterization

  • Serena DantiAffiliated withCUCCS/RRMR (Centro per l’Uso Clinico delle Cellule Staminali/Rete Regionale di Medicina Rigenerativa), University of PisaDepartment of Oncology, Transplants and Advanced Technologies, University of Pisa Email author 
  • , Cesare StefaniniAffiliated withCRIM Lab (Center of Research In Microengineering), Scuola Superiore Sant’Anna
  • , Delfo D’AlessandroAffiliated withCUCCS/RRMR (Centro per l’Uso Clinico delle Cellule Staminali/Rete Regionale di Medicina Rigenerativa), University of PisaDepartment of Human Morphology & Applied Biology, University of Pisa
  • , Stefania MoscatoAffiliated withDepartment of Human Morphology & Applied Biology, University of Pisa
  • , Andrea PietrabissaAffiliated withDepartment of Oncology, Transplants and Advanced Technologies, University of Pisa
  • , Mario PetriniAffiliated withCUCCS/RRMR (Centro per l’Uso Clinico delle Cellule Staminali/Rete Regionale di Medicina Rigenerativa), University of PisaDepartment of Oncology, Transplants and Advanced Technologies, University of Pisa
  • , Stefano BerrettiniAffiliated withDepartment of Neuroscience, ENT Unit, University of Pisa

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Abstract

Alternatives for ossicular replacements were fabricated in order to overcome persisting rejections in middle ear prosthetization. Unlike the synthetic prostheses in fashion, we propose biological and biohybrid replacements containing extra cellular matrix (ECM) molecules to improve biointegration. In this study, ECM-containing devices shaped as Partial Ossicular Replacement Prostheses (PORPs) were fabricated reproducing the current synthetic models. Biological PORPs were obtained from human decellularized cortical bone allografts by computer numerically controlled ultraprecision micromilling. Moreover, porous PORP-like scaffolds were produced and cultured with osteoinduced human mesenchymal stromal cells to generate in vitro bone ECM within the scaffold porosity (biohybrid PORPs). The acoustic responses of such devices were investigated and compared to those of commercial prostheses. Results showed that biological PORPs transmit mechanical signals with appropriate frequencies, amplitudes, and with early extinction time. Although signal transmission in biohybrid PORPs showed insufficient amplitude, we believe that tissue engineered constructs represent the new challenge in ossiculoplasty.

Keywords

Partial Ossicular Replacement Prosthesis (PORP) Middle ear Bone graft Tissue engineering Mesenchymal Stem Cell (MSC) Acoustic features