Biomedical Microdevices

, Volume 11, Issue 3, pp 609–614

Single particle adsorbing transfer system

Authors

  • Daniela Woide
    • Helmholtz Zentrum Munich, German Research Center for Environmental Health (GmbH), Institute of Radiation Protection
  • Veronika Mayer
    • Helmholtz Zentrum Munich, German Research Center for Environmental Health (GmbH), Institute of Radiation Protection
  • Thorsten Wachtmeister
    • Helmholtz Zentrum Munich, German Research Center for Environmental Health (GmbH), Institute of Radiation Protection
  • Norbert Hoehn
    • Xyz high precision
  • Albert Zink
    • EURAC, Institute for the iceman
  • Udo Koehler
    • Medizinisch Genetisches Zentrum
    • Helmholtz Zentrum Munich, German Research Center for Environmental Health (GmbH), Institute of Radiation Protection
Article

DOI: 10.1007/s10544-008-9270-8

Cite this article as:
Woide, D., Mayer, V., Wachtmeister, T. et al. Biomed Microdevices (2009) 11: 609. doi:10.1007/s10544-008-9270-8

Abstract

Here we present a novel approach for horizontal transfer of single particles after laser microdissection. The developed technique is a single particle adsorbing system for highly selective and gentle horizontal transfer of microdissected fixed and living material. As mediated via low-pressure technology, the transfer process can be precisely controlled, thus facilitating horizontal particle transfer of any isolated material, e.g. tissue material, single cells or chromosomes, in addition to precise positioning for sample release. This collection method allows one to predefine target positions and enables material transfer without contamination to any planar microchip device. This contamination free transfer is indispensable for novel lab-on-a-chip systems performing nanoscale polymerase chain reaction analyses. Using virtual reaction chamber microdevices, small amounts of microdissected material—as little as one single cell—can be directly transmitted and immediately used for single cell analysis.

Keywords

Laser microdissectionSingle particle handlingLow pressureLV-PCR microchip

Copyright information

© Springer Science+Business Media, LLC 2008