Article

Biomedical Microdevices

, Volume 11, Issue 2, pp 339-350

Magnetic-bead-based microfluidic system for ribonucleic acid extraction and reverse transcription processes

  • Chien-Ju LiuAffiliated withDepartment of Engineering Science, National Cheng Kung University
  • , Kang-Yi LienAffiliated withInstitute of Nanotechnology and Microsystems Engineering, National Cheng Kung University
  • , Ching-Yi WengAffiliated withThe Institute of Basic Medical Sciences, National Cheng Kung University
  • , Jyh-Wei ShinAffiliated withDepartment of Parasitology, National Cheng Kung University
  • , Tsuey-Yu ChangAffiliated withDepartment of Parasitology, National Cheng Kung University
  • , Gwo-Bin LeeAffiliated withDepartment of Engineering Science, National Cheng Kung UniversityInstitute of Nanotechnology and Microsystems Engineering, National Cheng Kung UniversityMedical Electronics and Device Technology Center, Industrial Technology Research Institute Email author 

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Abstract

This paper presents a new integrated microfluidic chip that automatically performs ribonucleic acid (RNA) extraction and reverse transcription (RT) processes. The microfluidic system consists of a microfluidic control module and a magnetic bio-separator. The microfluidic control module can perform pumping and mixing of small amount of fluids and subsequent purification and concentration of RNA samples by incorporating with the magnetic bio-separator consisting of 2-dimension twisted microcoils. Notably, the magnetic bio-separators are developed either to generate the required magnetic field to perform the separation of magnetic beads or to work as a micro-heater to control the temperature field for the following RT process. Experimental results show that the total RNA can be successfully purified and extracted by using magnetic beads and the subsequent RT processing of the RNA can be performed automatically. Total RNA is successfully extracted and purified from T98 cells utilizing the microfluidic system, which is comparable with the conventional methods. The whole automatic procedure of RNA sample extraction only takes 35 min, which is much faster than the conventional method (more than 2 h). As a whole, the developed microfluidic system may provide a powerful platform for rapid RNA extraction and RT processes for further biomedical applications.

Keywords

RNA extraction Reverse transcription Magnetic bead Microfluidics Magnetic separator μ-TAS MEMS