Biomedical Microdevices

, Volume 11, Issue 2, pp 323–329

Microfluidic aqueous two phase system for leukocyte concentration from whole blood

Authors

    • The Joint Department of Biomedical EngineeringNorth Carolina State University and the University of North Carolina at Chapel Hill
  • Glenn M. Walker
    • The Joint Department of Biomedical EngineeringNorth Carolina State University and the University of North Carolina at Chapel Hill
Article

DOI: 10.1007/s10544-008-9238-8

Cite this article as:
SooHoo, J.R. & Walker, G.M. Biomed Microdevices (2009) 11: 323. doi:10.1007/s10544-008-9238-8

Abstract

Leukocytes from a whole blood sample were concentrated using a microfluidic aqueous two phase system (μATPS). Whole blood was simultaneously exposed to polyethylene glycol (PEG) and dextran (Dex) phase streams and cells were partitioned based on their differential affinity for the streams. The laminar flow characteristic of microfluidic devices was used to create zero, one, and two stable interfaces between the polymer streams. Three different patterns of three polymer streams each were evaluated for their effectiveness in concentrating leukocytes: immiscible PEG-PEG-Dex, immiscible Dex-PEG-Dex, and miscible PEG-PBS-Dex. The most effective configuration was the Dex-PEG-Dex stream pattern which on average increased the ratio of leukocytes to erythrocytes by a factor of 9.13 over unconcentrated blood.

Keywords

Microfluidics Leukocyte Aqueous two phase separation Blood PEG Dex

Copyright information

© Springer Science+Business Media, LLC 2008