Article

Biomedical Microdevices

, Volume 10, Issue 5, pp 611-622

Nano-opto-mechanical characterization of neuron membrane mechanics under cellular growth and differentiation

  • Ashwini GopalAffiliated withDepartment of Biomedical Engineering, The University of Texas at Austin
  • , Zhiquan LuoAffiliated withDepartment of Mechanical Engineering, University of Texas at Austin
  • , Jae Young LeeAffiliated withDepartment of Chemical Engineering, University of Texas at Austin
  • , Karthik KumarAffiliated withDepartment of Biomedical Engineering, The University of Texas at Austin
  • , Bin LiAffiliated withDepartment of Mechanical Engineering, University of Texas at Austin
  • , Kazunori HoshinoAffiliated withDepartment of Biomedical Engineering, The University of Texas at Austin
  • , Christine SchmidtAffiliated withDepartment of Chemical Engineering, University of Texas at Austin
  • , Paul S. HoAffiliated withDepartment of Mechanical Engineering, University of Texas at Austin
  • , Xiaojing ZhangAffiliated withDepartment of Biomedical Engineering, The University of Texas at Austin Email author 

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Abstract

We designed and fabricated silicon probe with nanophotonic force sensor to directly stimulate neurons (PC12) and measured its effect on neurite initiation and elongation. A single-layer pitch-variable diffractive nanogratings was fabricated on silicon nitride probe using e-beam lithography, reactive ion etching and wet-etching techniques. The nanogratings consist of flexure folding beams suspended between two parallel cantilevers of known stiffness. The probe displacement, therefore the force, can be measured through grating transmission spectrum. We measured the mechanical membrane characteristics of PC12 cells using the force sensors with displacement range of 10 μm and force sensitivity 8 μN/μm. Young’s moduli of 425 ± 30 Pa are measured with membrane deflection of 1% for PC12 cells cultured on polydimethylsiloxane (PDMS) substrate coated with collagen or laminin in Ham’s F-12K medium. In a series of measurements, we have also observed stimulation of directed neurite contraction up to 6 μm on extended probing for a time period of 30 min. This method is applicable to measure central neurons mechanics under subtle tensions for studies on development and morphogenesis. The close synergy between the nano-photonic measurements and neurological verification can improve our understanding of the effect of external conditions on the mechanical properties of cells during growth and differentiation.

Keywords

Mechanotransduction Cytomechanics PC12 Cell membrane Growth Differentiation Nanogratings Micro-electro-mechanical systems (MEMS) Force sensor