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Nitric oxide acts downstream of hydrogen peroxide in the regulation of ascorbate and glutathione metabolism by jasmonic acid in Agropyron cristatum leaves

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Biologia Plantarum

Abstract

The relationship between hydrogen peroxide (H2O2) and nitric oxide (NO) in the regulation of ascorbate and glutathione metabolism by jasmonic acid (JA) in Agropyron cristatum leaves were studied. Results showed that JA increased the production of H2O2 and NO, the activities of ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), L-galactono-1,4-lactone dehydrogenase (GalLDH), and γ-glutamylcysteine synthetase (γ-ECS), as well as transcription of the respective genes and also the content of reduced ascorbate (AsA) and reduced glutathione (GSH). Above increases were suppressed by pre-treatments with H2O2 synthesis inhibitor diphenylene iodonium (DPI), H2O2 scavenger dimethylthiourea (DMTU), NO synthesis inhibitor N G-nitro-L-Arg methyl ester (L-NAME), and NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO). Pre-treatments with DPI and DMTU reduced H2O2 and NO production. Pre-treatments with L-NAME and cPTIO reduced NO production, but did not reduce the H2O2 production induced by JA. Our results suggested that NO acted downstream of H2O2 in JA signalling in the up-regulation of ascorbate and glutathione metabolism in A. cristatum leaves.

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Abbreviations

ABA:

abscisic acid

APX:

ascorbate peroxidase

AsA:

ascorbate

cPTIO:

2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide

DHAR:

dehydroascorbate reductase

DMTU:

dimethylthiourea

DPI:

diphenylene iodonium

GalLDH:

L-galactono-1,4-lactone dehydrogenase

γ-ECS:

γ-glutamylcysteine synthetase

GR:

glutathione reductase

GSH:

reduced glutathione

JA:

jasmonic acid

L-NAME:

N G-nitro-L-Arg methyl ester

MDHAR:

monodehydroascorbate reductase

SA:

salicylic acid

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Authors and Affiliations

  1. Henan Institute of Science and Technology, Xinxiang, 453003, P.R. China

    C. Shan & T. Yang

  2. Collaborative Innovation Center of Modern Biological Breeding, Xinxiang, 453003, P.R. China

    C. Shan & T. Yang

Authors
  1. C. Shan
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  2. T. Yang
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Correspondence to C. Shan.

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Acknowledgements: This study was supported by the Science and Technology Innovation Program of the Henan Institute of Science and Technology in 2011 and 2014.

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Shan, C., Yang, T. Nitric oxide acts downstream of hydrogen peroxide in the regulation of ascorbate and glutathione metabolism by jasmonic acid in Agropyron cristatum leaves. Biol Plant 61, 779–784 (2017). https://doi.org/10.1007/s10535-017-0708-9

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  • DOI: https://doi.org/10.1007/s10535-017-0708-9

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