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An intronless sucrose:fructan-6-fructosyltransferase (6-SFT) gene from Dasypyrum villosum enhances abiotic tolerance in tobacco

  • Original Paper
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Biologia Plantarum

Abstract

Fructans play vital roles in enhancing plant abiotic stress tolerance by reducing oxidative damage, stabilizing cell membranes, improving the osmotic adjustment capacity, and lowering the freezing point. In this study, a sucrose: fructan-6-fructosyltransferase (6-SFT) gene involved in the synthesis of fructans was isolated from Dasypyrum villosum, Dv-6-SFT, using genomic walking and reverse transcription (RT)-PCR. Alignment of the cDNA sequence with its genomic counterpart showed that no introns were present in the Dv-6-SFT gene, and thus it differs from all other plant 6-SFTs that have been cloned previously. Sequence analysis showed that the cDNA of the Dv-6-SFT sequence comprised 2 175 bp with a 1 863 bp open reading frame, and its deduced protein comprised 620 amino acids with a predicted molecular mass of 68.47 kDa. The Dv-6-SFT gene was transferred into tobacco (Nicotiana tabacum L.) cv. W38 via Agrobacterium-mediated transformation. The screened plants were tested by PCR and semi-quantitative RT-PCR, and the transgenic plants were evaluated under drought, cold, and salt stresses. The Dv-6-SFT transgenic tobacco plants had higher resistance to drought, cold, and salt stress than the non-transgenic plants. Further analysis showed that the transgenic plant expressing Dv-6-SFT had increased content of saccharides and proline, but reduced content of malondialdehyde in leaves. The results of this study demonstrate that the Dv-6-SFT gene is a potential candidate for conferring abiotic stress tolerance in plants and it could be used in crop improvement breeding programs.

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Abbreviations

1-FFT:

fructan:fructan 1-fructosyl transferase

cDNA:

complementary DNA

6G-FFT:

fructan:fructan 6G-fructosyltransferase

FT:

fructosyltransferase

GH32:

glycoside hydrolase family 32

gDNA:

genomic DNA

MDA:

malondialdehyde

MS:

Murashige and Skoog

NT:

non-transgenic

ORF:

open reading frame

pI:

isoelectric point

ROS:

reactive oxygen species

RT-PCR:

reverse transcription PCR

6-SFT:

sucrose:fructan-6-fructosyltransferase

1-SST:

sucrose:sucrose 1-fructosyltransferase

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Authors and Affiliations

  1. College of Agronomy, Northwest A&F University, Yangling, Shaanxi, 712100, P.R. China

    X. L. He, Z. Z. Chen, J. Wu, J. X. Zhao, J. N. Su, Z. H. Wang & X. H. Chen

  2. Shaanxi Key Laboratory of Genetic Engineering for Plant Breeding, Yangling, Shaanxi, 712100, P.R. China

    X. L. He & X. H. Chen

  3. College of Environment and Life Science, Kaili University, Kaili, GuiZhou, 556011, P.R. China

    X. L. He & J. W. Wang

  4. Institute for Wheat Research, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, P.R. China

    W. X. Li

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Acknowledgments: The authors thank Dr. D.E. Jackson for useful advice and editing the English language content of the manuscript. Much appreciated financial support was provided by the National Natural Science Foundation of China (31571650) and the Tang Zhong-Ying Breeding Funding Project of the Northwest A & F University.

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He, X.L., Wang, J.W., Li, W.X. et al. An intronless sucrose:fructan-6-fructosyltransferase (6-SFT) gene from Dasypyrum villosum enhances abiotic tolerance in tobacco. Biol Plant 61, 235–245 (2017). https://doi.org/10.1007/s10535-016-0696-1

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