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Cloning, characterization, and subcellular localization of a novel JAZ repressor from Eleusine coracana

  • Original Paper
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Biologia Plantarum

Abstract

Jasmonate ZIM domain (JAZ) proteins are key regulators of the jasmonic acid (JA) signaling pathway. Repressors of JAZ remain bound to the myelocytomatosis 2 (MYC2) or MYC3/MYC4 transcription factors in the absence of JA and negatively regulate transcription of the JA responsive genes. In the presence of JA, JAZ proteins interact with coronative insensitive 1 (COI1), the recognition molecule of E3 ubiquitin ligase SCFCOI1 (COI1 stabilized by Skp, cullin, F-box containing complex), get ubiquitinated, and subsequently degraded by the 26S proteasome. However, there is a dearth of knowledge about this gene family in monocot cereals, specifically its role in finger millet is unknown till date. Here we present the isolation and characterization of a novel JAZ family repressor gene from nonsequenced Eleusine coracana (EcJAZ) utilizing available genome information of Oryza, Sorghum, and Setaria. The EcJAZ sequence showed the presence of a conserved ZIM domain, the Jas motif, and N-terminal motif 7 like other Group1 TIFY sequence containing proteins. We observed coronatine (an analog of JA-Ile) dependent and time dependent degradation of recombinant EcJAZ that thereby fulfilled the basic characteristic of the JAZ proteins. We found a proteasome inhibitor N-(phenylmethoxy) carbonyl-L-leucyl-N-[(1R)-1-formyl-3-methylbutyl]-L-leucinamide) (MG132) mediated degradation inhibition of EcJAZ that supported its 26S proteasome mediated degradation. Our study shows the nuclear localization of GFP-EcJAZ by Agrobacterium mediated transient transformation of onion scale epidermal cells. In Eleusine leaves, transcription of EcJAZ increased 4.2-fold by salt stress and 5.5-fold by coronatine application; thus ascertained its inducibility by the abiotic stress as well as by bioactive JA-Ile. Taken together, all these results contribute to our understanding of the JA signaling pathway in Eleusine coracana.

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Abbreviations

ABA:

abscisic acid

COI1:

coronatine insensitive 1

GFP:

green fluorescence protein

Ile:

isoleaucine

JA:

jasmonic acid

JAZ:

jasmonate ZIM domain

MeJa:

methyl jasmonate

MG132:

N-(phenylmethoxy) carbonyl-L-Leucyl-N-[(1R)-1-formyl-3-methylbutyl]-L-leucinamide

MYC:

myelocytomatosis

RE:

restriction endonuclease

SCFCOI1 :

COI1 stabilized by Skp, Cullin, F-box containing complex

TIFY:

conserved TIFY sequence containing protein

ZIM:

zinc finger protein expressed in inflorescence meristem

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  1. Drug Development/Diagnostics and Biotechnology Division, CSIR - Indian Institute of Chemical Biology, Kolkata, 700032, India

    S. Sen & S. K. Dutta

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  1. S. Sen
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Correspondence to S. Sen.

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Acknowledgement: This study was supported by the WOS-A scheme of the Department of Science and Technology, the Government of India (Grant No. SR/WOS-A/LS-431/2011(G)). The authors are thankful to the Director, CSIR-IICB, for kindly allowing us to carry out this work. The authors are also thankful to Dr. S. Chattopadhyay for kindly providing pCAMBIA1303.

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Sen, S., Dutta, S.K. Cloning, characterization, and subcellular localization of a novel JAZ repressor from Eleusine coracana . Biol Plant 60, 715–723 (2016). https://doi.org/10.1007/s10535-016-0652-0

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