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Identification and characterization of a novel iron deficiency and salt stress responsive transcription factor IDEF1 in Porteresia coarctata

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Biologia Plantarum

Abstract

Iron availability affects plant growth depending on soil type. Mangroves are characterized by alkaline soils in which the halophytic wild rice relative Porteresia coarctata thrives. Young plants of P. coarctata grew optimally in the presence of 150 mM NaCl in a hydroponic medium and tolerated iron deficiency and salt up to 21 d without showing any symptoms of stress. A homolog of the rice iron deficiency responsive cis-acting element binding factor (IDEF1) that functions at the base of an iron regulated network was isolated and characterized from P. coarctata. PcIDEF1 had a close paralog in P. coarctata genome and its transcript expression was upregulated by both iron deficient conditions and salt treated conditions for up to three weeks. Sub-cellular localization study suggests nuclear targeting PcIDEF1 protein in guard cells and root tissues of tobacco. In vitro assays for metal binding affinity and binding PcIDEF1 to iron deficiency responsive element 1 (IDE1)-like elements in the 5’ flanking region of an iron regulated transporter from P. coarctata suggest that PcIDEF1 could potentially sense iron content in a plant cell and regulate expression of iron responsive genes containing IDE1-like elements in their promoter region. This study provides evidence for a possible cross-talk between iron deficiency and salt responses.

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Abbreviations

BPDS:

batho-phenanthroline disulphonic acid

dI-dC:

deoxyinosinic-deoxycytidylic

EMSA:

electrophoretic mobility shift assay

FRO:

ferric chelate reductase

GFP:

green fluorescent protein

His-6:

hexa-histidine

HN:

histidine-asparagine

IDE:

iron deficiency responsive element

IMAC:

immobilized metal ion affinity chromatography

MS:

Murashige and Skoog

ORF:

open reading frame

PcIDEF1 :

Porteresia coarctata iron deficiency responsive cis-acting element binding factor 1

PcIRT1 :

Porteresia coarctata iron regulated transporter 1

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  1. M.S. Swaminathan Research Foundation, Chennai, 600113, Tamilnadu, India

    D. Purohit, H. M. Sankararamasubramanian, A. Kumar Pal & A. Kumar Parida

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  1. D. Purohit
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  2. H. M. Sankararamasubramanian
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Correspondence to A. Kumar Parida.

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Acknowledgement

The work presented in the manuscript was funded by the Department of Biotechnology, the Government of India. The clone for the green fluorescent protein (mGFP6) was kindly provided by Dr. Mark Curtis, the Institute of Plant Biology, Zurich, Switzerland. We also acknowledge the help of Dr. Sesha Bamini, the National Centre for Ultrafast Processing, Chennai, in confocal microscopy studies.

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Purohit, D., Sankararamasubramanian, H.M., Kumar Pal, A. et al. Identification and characterization of a novel iron deficiency and salt stress responsive transcription factor IDEF1 in Porteresia coarctata . Biol Plant 60, 469–481 (2016). https://doi.org/10.1007/s10535-016-0616-4

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