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Enhancement of antioxidant enzyme activities in rice callus by ascorbic acid under salinity stress

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Biologia Plantarum

Abstract

Ascorbic acid (AsA) is naturally occurring compound with antioxidant activity and plays a pivotal role in plant cell adaptation to salinity stress. The objective of this work was to assess the influence of exogenous AsA on the embryogenic callus of indica rice (Oryza sativa L.) cv. MRQ74 cultivated under saline conditions. NaCl (200 mM) decreased callus fresh and dry masses, relative growth rate, and K+ and Ca+2 content, and increased Na+ content and Na+/K+ ratio. Application of AsA (0.5 or 1 mM) alleviated these effects of salinity. Activities of peroxidase, catalase, superoxide dismutase, as well as content of proline increased due to the NaCl treatment, and these parameters were mostly further increased by 0.5 mM AsA. Thus, AsA can increase callus tolerance to NaCl stress.

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Abbreviations

ABA:

abscisic acid

APX:

ascorbate peroxidase

AsA:

ascorbic acid

BAP:

6-benzylaminopurine

CAT:

catalase

2,4-D:

2,4-dichlorophenoxyacetic acid

ET:

ethylene

GA3 :

gibberellic acid

JA:

jasmonic acid

MS:

Murashige and Skoog

POD:

peroxidase

PVP:

polyvinylpyrrolidone

RGR:

relative growth rate

ROS:

reactive oxygen species

SA:

salicylic acid

SOD:

superoxide dismutase

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Authors and Affiliations

  1. Faculty of Science and Technology, University of Kebangsaan, UKM, 43600, Bangim, Selangor, Malaysia

    A. N. Alhasnawi, C. M. Z. Che Radziah, A. A. Kadhimi, A. Isahak & W. M. W. Yusoff

  2. University Presidency, AL-Muthanna University, 60001, Samawah, Iraq

    A. N. Alhasnawi

  3. University of Baghdad, Ministry of Higher Education, Jadriyah, 10070, Baghdad, Iraq

    A. A. Kadhimi

  4. Malaysian Nuclear Agency, 43000, Kajang, Bangi, Malaysia

    A. Mohamad

Authors
  1. A. N. Alhasnawi
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  2. C. M. Z. Che Radziah
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  3. A. A. Kadhimi
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  4. A. Isahak
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  5. A. Mohamad
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  6. W. M. W. Yusoff
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Corresponding author

Correspondence to A. N. Alhasnawi.

Additional information

Acknowledgements: This study is a part of the Ph.D. programme of the first author. This project was financed by Al-Muthanna University, the Ministry of Higher Education and Scientific Research, Iraq, the University Kebangsaan, Malaysia, and the Ministry of Higher Education, Malaysia.

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Alhasnawi, A.N., Che Radziah, C.M.Z., Kadhimi, A.A. et al. Enhancement of antioxidant enzyme activities in rice callus by ascorbic acid under salinity stress. Biol Plant 60, 783–787 (2016). https://doi.org/10.1007/s10535-016-0603-9

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  • DOI: https://doi.org/10.1007/s10535-016-0603-9

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