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Analysis of the flanking sequences of the heterochromatic JNK region in Secale vavilovii chromosomes

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Biologia Plantarum

Abstract

The JNK motif is repeated 4 000 times in an additional heterochromatin band. Flanking sequences of a JNK region, which form extra heterochromatin in 2R rye (Secale vavilovii Grossh.) chromosomes, were studied using a genome walking technique. The results clearly indicate that there were blocks of JNK sequences adjacent to the R173 family of repeated sequences. Moreover, it appears that the R173 are sequences flanking in both directions, i.e., upstream and downstream. Downstream, the R173 is adjacent to the JNKs in an anti-parallel orientation, whereas upstream it is adjacent in a parallel orientation. In order to confirm the presence of the R173 sequence, fluorescence in situ hybridization was carried out. Using both JNK and R173 molecular probes, overlapping hybridization signals in the 2RL pair of chromosomes were observed, indicating an identical location of the two sequence elements.

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Abbreviations

DAPI:

4′,6-diamidino-2-phenylindole

FISH:

fluorescence in situ hybridization

SSC:

saline sodium citrate

TBE:

Tris-borate-EDTA

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Authors and Affiliations

  1. Department of Cell Biology, University of Szczecin, Wąska 13, PL-71415, Szczecin, Poland

    A. Kalinka & M. Achrem

  2. Molecular Biology and Biotechnology Center, Faculty of Biology, University of Szczecin, Wąska 13, PL-71415, Szczecin, Poland

    A. Kalinka & M. Achrem

Authors
  1. A. Kalinka
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  2. M. Achrem
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Correspondence to M. Achrem.

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Acknowledgments: This work was financially supported by the National Science Centre, Poland (Grant No. NN 310 436038).

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Kalinka, A., Achrem, M. Analysis of the flanking sequences of the heterochromatic JNK region in Secale vavilovii chromosomes. Biol Plant 59, 637–644 (2015). https://doi.org/10.1007/s10535-015-0531-0

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  • DOI: https://doi.org/10.1007/s10535-015-0531-0

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