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Alterations in the porphyrin biosynthesis and antioxidant responses to chilling and heat stresses in Oryza sativa

  • Original Papers
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Biologia Plantarum

Abstract

Roles of an altered porphyrin biosynthesis and antioxidants in protection against chilling and heat stresses were evaluated in rice (Oryza sativa L.). When exposed to the same exposure time (6 or 30 h), heat-stressed (45 °C) plants exhibited a less oxidative stress as indicated by a lower dehydration, ion leakage, and H2O2 production compared to chilling-stressed (4 °C) plants. Malondialdehyde production also increased after a mild chilling stress, whereas it increased only after a long-term heat stress. The content of protoporphyrin IX, Mg-protoporphyrin IX and its methyl ester, and protochlorophyllide drastically declined under both the stresses, particularly under the long-term heat stress. Greater increases in catalase and peroxidase activities in heat-stressed plants indicate more cofactors supplied for hemoproteins compared to those of chilling-stressed and untreated control plants. Intermediates of carotenoid biosynthesis, zeaxanthin and antheraxanthin, also increased under the chilling and heat stresses. In comparison to chilling-stressed plants, heat-stressed plants were more efficient in porphyrin scavenging and antioxidant enzyme responses, which may play crucial roles in plant protection under temperature stress, thereby suffering less from oxidative stress.

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Abbreviations

ALA:

5-aminolevulinic acid

APX:

ascorbate peroxidase

CAT:

catalase

DAB:

3,3-diaminobenzidine

MDA:

malondialdehyde

MgProto IX:

Mg-protoporphyrin IX

MgProto IX ME:

Mg-protoporphyrin IX methyl ester

Pchlide:

protochlorophyllide

POD:

peroxidase

Proto IX:

protoporphyrin IX

ROS:

reactive oxygen species

RWC:

relative water content

SOD:

superoxide dismutase

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Authors and Affiliations

  1. School of Life Sciences and Biotechnology, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu, 702-701, Korea

    T. -H. Phung & S. Jung

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  1. T. -H. Phung
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  2. S. Jung
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Correspondence to S. Jung.

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Acknowledgements: This work was supported by the National Research Foundation of Korea Grant funded by the Ministry of Education, Science and Technology (NRF-2009-0076123 and NRF-2010-0005635).

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Phung, T.H., Jung, S. Alterations in the porphyrin biosynthesis and antioxidant responses to chilling and heat stresses in Oryza sativa . Biol Plant 59, 341–349 (2015). https://doi.org/10.1007/s10535-015-0505-2

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  • DOI: https://doi.org/10.1007/s10535-015-0505-2

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