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Effect of ochratoxin A and buthionine sulfoximine on proteome and ascorbate-glutathione cycle enzymes in Arabidopsis thaliana

  • Original Papers
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Biologia Plantarum

Abstract

In this study, proteome and activities of glutathione (GSH)-related enzymes were investigated in detached leaves of Arabidopsis thaliana treated with ochratoxin A (OTA) alone or supplemented with buthionine sulfoximine (BSO, a specific inhibitor of the first step in GSH biosynthesis). A comparative proteomic study using two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption ionization-time of flight tandem mass spectrometry (MALDI-TOF/TOF MS/MS) identified 12 differentially expressed proteins mainly involved in GSH metabolism, energy metabolism, sugar metabolism, and photosynthesis. The treatment with OTA significantly enhanced the activities of glutathione-S-transferase (GST) and glutathione reductase (GR) through up-regulating the corresponding genes (GSTF7, GR1), an the diminishing effect of BSO on them counteracted the results. However, both OTA and BSO decreased the activity of ascorbate peroxidase (APX), and OTA also decreased the monodehydroascorbate reductase (MDHAR) and glutathione peroxidase (GPX) activities. Briefly, the OTA-induced phytotoxicity to the A. thaliana detached leaves was increased slightly by addition of BSO, and the fluctuation in GSH synthesis, GSH metabolism and disorder of cellular metabolism happened.

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Abbreviations

APX:

ascorbate peroxidases

ASH:

reduced ascorbate

AsA:

ascorbate

BSO:

buthionine sulfoximine

DGE:

digital gene expression

DHA:

dehydroascorbic acid

DHAR:

dehydroascorbate reductase

GSH:

reduced glutathione

GPX:

glutathione peroxidases

GR:

glutathione reductase

GSH-S:

glutathione synthase

GS2:

glutamine synthetase 2

GSSG:

oxidized glutathione

GST:

glutathione-S-transferase

HCF136:

high chlorophyll fluorescence 136

HR:

hypersensitive response

MDHAR:

monodehydroascorbate reductase

OTA:

ochratoxin A

PCD:

programmed cell death

ROS:

reactive oxygen species

TIM:

triosephosphate isomerase

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Authors and Affiliations

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, P.R. China

    J. R. Hao, Y. Wang, W. W. Zhao, W. T. Xu, Y. B. Luo, W. H. Wu & K. L. Huang

  2. The Supervision, Inspection and Testing Center of Genetically Modified Organisms, Beijing, 100083, P.R. China

    W. T. Xu, Y. B. Luo, Z. J. Yang, Z. H. Liang & K. L. Huang

  3. Beijing Key Laboratory of Nutrition, Health and Food Safety, Beijing, 100083, P.R. China

    Z. J. Yang & W. H. Wu

  4. Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, P.R. China

    Y. Wang

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  1. J. R. Hao
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  2. Y. Wang
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  3. W. W. Zhao
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  4. W. T. Xu
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  9. K. L. Huang
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Correspondence to W. T. Xu.

Additional information

Acknowledgements: We thank all members of the Kunlun Huang's Laboratory who contributed to the project, the mass spectrometry support from the Beijing Proteome Research Center and the Support Projects (D121100003112001, D121100003112004). The first two authors contributed equally to this work.

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Hao, J.R., Wang, Y., Zhao, W.W. et al. Effect of ochratoxin A and buthionine sulfoximine on proteome and ascorbate-glutathione cycle enzymes in Arabidopsis thaliana . Biol Plant 59, 331–340 (2015). https://doi.org/10.1007/s10535-015-0492-3

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  • DOI: https://doi.org/10.1007/s10535-015-0492-3

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