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Responses of two barley cultivars differing in their salt tolerance to moderate and high salinities and subsequent recovery

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Biologia Plantarum

Abstract

Two barley (Hordeum vulgare L.) cultivars, Czech spring cv. Amulet and Syrian landrace Tadmor, were subject to different salinity treatments: 1) the NaCl concentration was gradually increased from 0 (the control) to either 100 (a moderate salt stress) or 300 mM NaCl (a high salt stress), 2) the NaCl concentration was increased directly either from 0 to 300 mM NaCl or from 100 to 300 mM NaCl, and 3) a recovery when all variants were transferred back to control conditions and cultivated for seven additional days before sampling. The following parameters were determined: water saturation deficit (WSD), osmotic potential (ψs), leaf proline content, maximum quantum yield of photosystem (PS) II photochemistry (measured as variable to maximum chlorophyll a fluorescence ratio, Fv/Fm), and relative accumulation of dehydrins (DHN). Both quantitative and qualitative differences in dehydrins were found between NaCl-treated Amulet and Tadmor. A principal component analysis (PCA) of all experiment data revealed a differential ability of Amulet and Tadmor to recover after the 300 mM NaCl treatments indicating better salt tolerance in Tadmor. Correlation analyses have shown statistically significant correlations between WSD, ψs, proline, and DHN.

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Abbreviations

DHN:

dehydrins

Fv/Fm :

variable to maximum chlorophyll a fluorescence ratio

PCA:

principal component analysis

PS:

photosystem

WSD:

water saturation deficit

ψs :

osmotic potential

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Authors and Affiliations

  1. Department of Plant Genetics, Breeding and Crop Quality, Crop Research Institute, Drnovská 507, CZ-16106, Prague, Czech Republic

    K. Kosová, P. Vítámvás, M. O. Urban, E. Vlasáková & I. T. Prášil

  2. Department of Biochemistry and Microbiology, Institute of Chemical Technology Prague, Technická 5, CZ-16628, Prague, Czech Republic

    I. Hlaváčková

Authors
  1. K. Kosová
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  2. P. Vítámvás
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  3. I. Hlaváčková
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  4. M. O. Urban
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  5. E. Vlasáková
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  6. I. T. Prášil
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Correspondence to K. Kosová.

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Acknowledgements: The work was supported by the Czech Ministry of Education No. LD11069 as part of COST action FA0901 and by the Czech Ministry of Agriculture No. MZe RO0414. The authors thank Dr. Peter Lemkin for a linguistic revision of the whole manuscript.

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Kosová, K., Vítámvás, P., Hlaváčková, I. et al. Responses of two barley cultivars differing in their salt tolerance to moderate and high salinities and subsequent recovery. Biol Plant 59, 106–114 (2015). https://doi.org/10.1007/s10535-014-0465-y

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  • DOI: https://doi.org/10.1007/s10535-014-0465-y

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