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Application of trehalose ameliorates heat stress and promotes recovery of winter wheat seedlings

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  • Published:
Biologia Plantarum

Abstract

Trehalose was supplied to wheat (Triticum aestivum L.) seedlings just before a high temperature (40 °C) treatment and some physiological parameters were measured during the heat stress and recovery. The application of trehalose decreased the net photosynthetic rate (PN) of wheat seedlings under the heat stress, but to a small extent increased the dry mass (DM) and leaf water content (LWC) after recovery from the heat stress. The trehalose-induced decrease in PN under the heat stress was not associated with a stomatal response. The heat stress slightly decreased the maximal efficiency of photosystem II (PS II) photochemistry (the variable to maximum chlorophyll a fluorescence ratio, Fv/Fm) similarly in the trehalose treated or non-treated plants. Under the heat stress, the actual efficiency of PS II photochemistry (ΦPSII) and the efficiency of excitation energy capture by open reaction centers (Fv′/Fm′) were lower in the trehalose-pretreated seedlings, whereas they were higher after the recovery. The patterns of changes in nonphotochemical quenching (NPQ) were contrary to those of ϕPS II and Fv′/Fm′. The chlorophyll content was lower, whereas the β-carotene content and the degree of de-epoxidation (DEPS) of xanthophyll cycle pigments were higher in the trehalose-pretreated wheat seedlings under the heat stress. These results suggest that exogenous trehalose partially promotes recovery of wheat by the increase of NPQ, β-carotene content, and DEPS.

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Abbreviations

A:

antheraxanthin

Car:

β-carotene

Chl:

chlorophyll

DEPS:

degree of deepoxidation [(A+Z)/(V+A+Z)]

Fv/Fm :

variable to maximum Chl fluorescence ratio in dark adapted leaves (the maximal efficiency of PS II photochemistry)

Fv′/Fm′:

variable to maximum Chl fluorescence ratio under steady-state conditions (the efficiency of excitation energy capture by open reaction centers)

gs :

stomatal conductance

LWC:

leaf water content

NPQ:

non-photochemical quenching

PN :

net photosynthetic rate

PS:

photosystem

qp :

photochemical quenching

V:

violaxanthin

Z:

zeaxanthin

ϕPS II :

the actual efficiency of PS II photochemistry

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Authors and Affiliations

  1. Instruments Sharing Platform of School of Life Sciences, East China Normal University, Shanghai, 200241, China

    Y. Luo, Y. M. Gao & C. J. Zou

  2. School of Life Sciences, Shandong Agricultural University, Taian, 271018, China

    W. Wang

  3. State Key Laboratory of Crop Biology, Taian, 271018, China

    W. Wang

Authors
  1. Y. Luo
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  2. Y. M. Gao
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  3. W. Wang
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  4. C. J. Zou
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Correspondence to Y. Luo.

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Acknowledgements: This work was supported by the Natural Science Foundation of China (No. 31200301), and the Fund of State Key Laboratory of Crop Biology (No. 2013KF01).

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Luo, Y., Gao, Y.M., Wang, W. et al. Application of trehalose ameliorates heat stress and promotes recovery of winter wheat seedlings. Biol Plant 58, 395–398 (2014). https://doi.org/10.1007/s10535-014-0397-6

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  • DOI: https://doi.org/10.1007/s10535-014-0397-6

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