Abstract
An AREB gene, designated as AhAREB1, was cloned from peanut (Arachis hypogaea L.). The gene contains a 1 338-bp open reading frame that encodes a putative protein of 445 amino acids. The corresponding genomic DNA containing four exons and three introns was isolated and analyzed. An upstream 1 060-bp DNA promoter fragment of the AhAREB1 gene was also amplified from peanut genomic DNA. Multiple sequence alignment of the deduced amino acids of AREB showed that the AhAREB1 protein shares high sequence homology with GmAREB1, S1AREB and ABF2. Quantitative real-time PCR analysis showed that AhAREB1 was induced by polyethylene glycol, NaCl, gibberellic acid, abscisic acid and salicylic acid. The cloning and characterization of the AhAREB1 gene will be useful for further studies establishing the biological role of AhAREB1 in plants.
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Abbreviations
- ABA:
-
abscisic acid
- ABF:
-
ABRE binding factor
- ABRE:
-
ABA response element
- AREB:
-
ABA-response element binding protein
- bZIP:
-
basic leucine zipper
- CaMK:
-
calmodulin-dependent protein kinase
- CK:
-
casein kinase
- GA3 :
-
gibberellic acid
- NCED:
-
9-cis-epoxycarotenoid dioxygenase
- ORF:
-
open reading frame
- PEG:
-
polyethylene glycol
- PKC:
-
protein kinase C
- SA:
-
salicylic acid
- W-BOX:
-
WRKY binding site
- WRKY:
-
superfamily of plant transcription factors
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Acknowledgments: We thank Drs. Nicole Grant, Katia Silvera and Louis Santiago for critical readings of the manuscript. This work was supported by National Natural Science Foundation of China (30971715 and 31171468 to Ling Li), Guangdong Natural Science Foundation (915106310100001 and 10251063101000010 to Ling Li), and China Postdoctoral Science Foundation (20090460783 to Dr. Lan Hong). The first two authors contributed equally to this study.
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Hong, L., Hu, B., Liu, X. et al. Molecular cloning and expression analysis of a new stress-related AREB gene from Arachis hypogaea . Biol Plant 57, 56–62 (2013). https://doi.org/10.1007/s10535-012-0236-6
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DOI: https://doi.org/10.1007/s10535-012-0236-6