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Variability for resistance to Fusarium solani culture filtrate and fusaric acid among somaclones in pea

  • Published:
Biologia Plantarum

Abstract

Pea (Pisum sativum L.) somaclones of cultivars Adept, Komet and Bohatýr were obtained after selection in vitro with Fusarium solani filtrate and fusaric acid (FA). R2 regenerants were analysed by random amplification of polymorphic DNA (RAPD; OPAB4, P-14, UBC-556) and inter-retrotransposon amplification polymorphism (IRAP; Ogre) markers. Marker UBC-556 showed different banding patterns for each cultivar, but without specific bands for selected and control plants. Markers OPAB4, P14 and Ogre were useful for clear discrimination between selected and non-selected variants of all three cultivars. Flow cytometry analysis proved the same genome size of selected and non-selected pea lines. Therefore in vitro selection by pathogen derived agents could be the efficient method for obtaining of pea somaclones with increased resistance to F. solani.

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Abbreviations

BAP:

benzylaminopurine

FA:

fusaric acid

IRAP:

inter-retrotransposon amplification polymorphism

ISSR:

intersimple sequence repeat

NAA:

naphthaleneacetic acid

RAPD:

random amplification of polymorphic DNA

References

  • Bacon, C.W., Porter J.K., Norred, W.P., Leslie, F.J.: Production of fusaric acid by Fusarium species. — Appl. environ. Microbiol. 62: 4039–4043, 1996.

    PubMed  CAS  Google Scholar 

  • Coyne, C.J., McClendon, M.T., Walling, J.G., Timmermann-Vaughan, G.M., Murray, S., Meksem, K., Lightfoot, D.A., Schultz, J.L., Keller, K.E., Martin, R.R., Inglis, D.A., Rajesh, P.N., McPhee, K.E., Weeden, N.F., Grusa, K M.A., Li, C.M., Storlie, E.W.: Construction and characterization of two bacterial artificial chromosome libraries of pea (Pisum sativum L.) for the isolation of economically important genes. — Genome 50: 871–875, 2007.

    Article  PubMed  CAS  Google Scholar 

  • Doležel, J., Binarová, P., Lucretti, S.: Analysis of nuclear DNA content in plant cells by flow cytometry. — Biol. Plant. 31: 113–120, 1989.

    Article  Google Scholar 

  • Freytag, A.H., Rao-Arelli, A.P., Anand, S.C., Wrather, J.A., Owens, L.D.: Somaclonal variation in soybean plants regenerated from tissue culture. — Plant Cell Rep. 8: 199–202, 1989.

    Article  Google Scholar 

  • Galbraith, D.W., Harkins, K.R., Maddox, J.R., Ayres, N.M., Sharma, D.P., Firoozabady, E.: Rapid flow cytometric analysis of the cell-cycle in intact plant-tissues. — Science 220: 1049–1051, 1983.

    Article  PubMed  CAS  Google Scholar 

  • Gamborg, O.L., Miller, R.A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells. — Exp. Cell Res. 50: 151, 1968.

    Article  PubMed  CAS  Google Scholar 

  • Gesteira, A.S., Otoni, W.C., Barros, E.G., Moreira, M.A.: RAPD-based detection of genomic instability in soybean plants derived from somatic embryogenesis. — Plant Breed. 121: 269–271, 2002.

    Article  CAS  Google Scholar 

  • Greilhuber, J., Doležel, J., Lysák, M., Bennett, M.D.: The origin, evolution and proposed stabilisation of the terms “genome size“ and “C-value“ to describe DNA contents. — Ann. Bot. 95: 255–260, 2005.

    Article  PubMed  CAS  Google Scholar 

  • Griga, M., Tejklová, E., Novák, F.J., Kubaláková, M.: In vitro clonal propagation of Pisum sativum L. — Plant Cell Tissue Organ Cult. 6: 95–104, 1986.

    Article  CAS  Google Scholar 

  • Hess, M., Barralis, G., Bleiholder, H., Buhr, L., Eggers, T.H., Hack, H., Stauss, R.: Use of the extended BBCH scale — general for the descriptions of the growth stages of mono- and dicotyledonous weed species. — Weed Res 37: 433–441, 1997.

    Article  Google Scholar 

  • Hirochika, H.: Activation of tobaco retrotransposons during tissue culture. — EMBO J. 12: 2521–2528, 1993.

    PubMed  CAS  Google Scholar 

  • Horáček, J., Griga, M., Smýkal, P., Hýbl, M.: Effect of environmental and genetic factors on the stability of pea (Pisum sativum L.) isozyme and DNA markers. — Czech J. Genet. Plant Breed. 45: 57–71, 2009.

    Google Scholar 

  • Kalendar, R., Grob, T., Regina, M., Suoniemi, A., Schulman, A.: IRAP and REMAP: two new retrotransposon-based DNA fingerprinting techniques. — Theor. appl. Genet. 98: 704–711, 1999.

    Article  CAS  Google Scholar 

  • Kuzniak, E.: Effect of fusaric acid on reactive oxygen species and antioxidants in tomato cell cultures. — J. Phytopathol. 149: 575–582, 2001.

    Article  CAS  Google Scholar 

  • Larkin, P.J., Scowcroft, W.R.: Somaclonal variation a novel source of variability from cell cultures for plant improvement. — Theor. appl. Genet. 60: 443–455, 1981.

    Article  Google Scholar 

  • Lebeda, A., Švábová, L.: In vitro screening methods for assessing of plant disease resistance. — In: Spencer, M.M., Lebeda, A. (ed.): Mass Screening Techniques for Selecting Crops Resistant to Diseases. Pp. 5–45. IAEA, Vienna 2010

    Google Scholar 

  • Loureiro, J., Suda, J., Doležel, J., Santos, C.: FLOWer: a plant DNA flow cytometry database. — In: Doležel, J., Greilhuber J., Suda J. (ed.): Flow Cytometry with Plant Cells. Pp. 423–438, Willey, Weinheim 2007.

    Chapter  Google Scholar 

  • Lysák, M.A., Doležel, J.: Estimation of nuclear DNA content in Sesleria (Poaceae). — Caryologia 51: 123–132, 1998.

    Article  Google Scholar 

  • Matsumoto, K., Barbosa, M.L., Souza, L.A.C., Teixeira, J.B.: Race 1 fusarium wilt tolerance on banana plants selected by fusaric acid. — Euphytica 84: 67–71, 1995.

    Article  Google Scholar 

  • Mc Clintock, B.: The significance of responses of the genome to challenge. — Science 226: 792–801, 1984.

    Article  CAS  Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures. — Physiol. Plant. 15: 472–497, 1962.

    Article  Google Scholar 

  • Neumann, P., Požárková, D., Macas, J.: Highly abundant pea LTR retrotransposons Ogre is constitutively transcribed and partially spliced. — Plant mol. Biol. 53: 399–410, 2003.

    Article  PubMed  CAS  Google Scholar 

  • Remotti, P.C., Löffler, H.J.M.: The involvement of fusaric acid in the bulb-rot of Gladiolus. — J. Phytopathol. 144: 405–411, 1996.

    Article  CAS  Google Scholar 

  • Rival, A., Bertrand, L., Beule, T., Combes, M.C., Troulot, P., Lashermes, P.: Suitability of RAPD analysis for the detection of somaclonal variants in oil palm (Elaeis guineensis Jasq.). — Plant Breed. 117: 73–76, 1998.

    Article  Google Scholar 

  • Sakker, M.M., Adawy, S.S., Mohamed, A.A., El-Itriby, H.A.: Monitoring of cultivar identitying tissue culture derived date palms using RAPD and AFLP analysis. — Biol. Plant. 50: 198–204, 2006.

    Article  Google Scholar 

  • Samec, P., Pošvec, Z., Stejskal, J., Našinec, V., Griga, M.: Cultivar identification and relationships in Pisum sativum L. based on RAPD and isoenzymes. — Biol. Plant. 41: 39–48, 1998.

    Article  CAS  Google Scholar 

  • Scowcroft, W.R.: Somaclonal variation: the myth of clonal uniformity. — In: Hohn, B., Dennis, E.B. (ed.): Genetic Flux in Plants. Pp. 217–245. Springer-Verlag, Berlin - Wien 1985.

    Chapter  Google Scholar 

  • Simioniuc, D., Uptmoor, R., Friedt, W. Ordon, F.: Genetic diversity and relationships among pea cultivars revealed by RAPDs and AFLPs. — Plant Breed. 121: 429–435, 2002.

    Article  CAS  Google Scholar 

  • Smýkal, P.: Development of an efficient retrotransposon-based fingerprinting method for rapid pea variety identification. — J. appl. Genet. 47: 221–230, 2006.

    Article  PubMed  Google Scholar 

  • Smýkal, P., Valledor, L., Rodríguez, R., Griga, M.: Assessment of genetic and epigenetic stabiltiy in long-term in vitro shoot culture of pea (Pisum sativum L.). — Plant Cell Rep. 26: 1985–1998, 2007.

    Article  PubMed  Google Scholar 

  • Sonyia, E.V., Banerjee, N.S., Das, M.R.: Genetic analysis of somaclonal variation among callus-derived plants of tomato. — Curr. Sci. 80: 1213–1215, 2001.

    Google Scholar 

  • Švábová, L., Lebeda, A.: In vitro selection for improved resistance to toxin-producing pathogens. — J. Phytopathol. 153: 52–64, 2005.

    Article  Google Scholar 

  • Švábová, L., Lebeda, A., Kitner, M., Sedlářová, M., Petřivalský, M., Dostálová, R., Ondřej, M., Horáček, J., Smýkalová I., Griga M.: Comparison of the effects of Fusarium solani filtrates in vitro and in vivo on morphological characteristics and peroxidase activity in pea cultivars with different susceptibility. — J. Plant Pathol. 93: 19–30, 2011.

    Google Scholar 

  • Wiesner, I., Wiesnerová, D., Pošvec, Z., Griga, M. Horáček, J.: Evaluation of pea somaclones by protein and DNA arkers. — In: Proc. 4th Europ. Conf. on Grain Legumes. Pp. 150–151. Trocadero, Paris - Cracow 2001.

    Google Scholar 

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Authors and Affiliations

  1. Biotechnology Department, Agritec Plant Research Ltd., Zemědělská 16, CZ-78701, Šumperk, Czech Republic

    J. Horáček & L. Švábová

  2. Faculty of Science, Department of Botany, Palacký University in Olomouc, Šlechtitelů 11, CZ-78371, Olomouc-Holice, Czech Republic

    P. Šarhanová & A. Lebeda

Authors
  1. J. Horáček
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  2. L. Švábová
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  3. P. Šarhanová
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  4. A. Lebeda
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Corresponding author

Correspondence to A. Lebeda.

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Acknowledgements: This research was supported by grants of Czech Ministry of Education, Youth and Sports MSM 6198959215, MSM 2678424601 and National Agency for Agricultural Research QG60099. The authors thank to Dr. Petr Smýkal for providing primers for RAPD and IRAP analyses.

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Horáček, J., Švábová, L., Šarhanová, P. et al. Variability for resistance to Fusarium solani culture filtrate and fusaric acid among somaclones in pea. Biol Plant 57, 133–138 (2013). https://doi.org/10.1007/s10535-012-0131-1

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  • DOI: https://doi.org/10.1007/s10535-012-0131-1

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