Biologia Plantarum

, Volume 55, Issue 2, pp 396–399

Isolation and characterization of eleven polymorphic microsatellite loci in Aegiphila sellowiana and their transferability

Authors

  • E. A. Ruas
    • Departamento de Agronomia, Centro de Ciências AgráriasUniversidade Estadual de Londrina
  • J. O. Damasceno
    • Departamento de Agronomia, Centro de Ciências AgráriasUniversidade Estadual de Londrina
  • A. R. O. Conson
    • Departamento de Biologia Geral, Centro de Ciências BiológicasUniversidade Estadual de Londrina
  • B. F. Costa
    • Departamento de Biologia Geral, Centro de Ciências BiológicasUniversidade Estadual de Londrina
  • L. A. Rodrigues
    • Departamento de Biologia Geral, Centro de Ciências BiológicasUniversidade Estadual de Londrina
  • M. Reck
    • Departamento de Biologia Geral, Centro de Ciências BiológicasUniversidade Estadual de Londrina
  • A. O. Santos Vieira
    • Departamento de Biología Animal e Vegetal, Centro de Ciências BiológicasUniversidade Estadual de Londrina
  • C. deF. Ruas
    • Departamento de Biologia Geral, Centro de Ciências BiológicasUniversidade Estadual de Londrina
  • C. Medri
    • Departamento de BiologiaUniversidade do Norte do Paraná, Bandeirantes
    • Departamento de Biologia Geral, Centro de Ciências BiológicasUniversidade Estadual de Londrina
Brief Communication

DOI: 10.1007/s10535-011-0063-1

Cite this article as:
Ruas, E.A., Damasceno, J.O., Conson, A.R.O. et al. Biol Plant (2011) 55: 396. doi:10.1007/s10535-011-0063-1

Abstract

We isolated and characterized eleven polymorphic microsatellite loci for Aegiphila sellowiana an outcrossing pioneer tree species that is frequently used in reforestation programs of tropical riparian forests in Brazil. A total of 38 alleles were detected across a sample of 45 individuals of A. sellowiana, with an average number of 3.45 alleles per locus. The average polymorphic information content (PIC) was 0.430 and the observed (HO) and expected (HE) heterozygosity values varied from 0.156 to 1.000 and 0.145 to 0.730, respectively. Eight loci exhibited significant deviation from Hardy-Weinberg equilibrium (P ≤ 0.001) and 32 pair combinations of loci showed significant linkage disequilibrium (P ≤ 0.001). All 11 primers were tested for cross amplification in 12 species belonging to the family Lamiaceae and 5 species belonging to the related family Verbenaceae. The sequence and diversity information obtained using these microsatellites and their cross-transferability to other species of Lamiaceae as well as Verbenaceae will increase our understanding of genetic structures and species relationships within Aegiphyla and other genera of these families.

Additional key words

cross amplificationgenetic diversityLamiaceaemicrosatellite primersVerbenaceae

Abbreviations

SSR

microsatellite or simple sequence repeat

CTAB

cetyltrimethylammonium bromide

PCR

polymerase chain reaction

Copyright information

© Springer Science+Business Media B.V. 2011