Skip to main content
SpringerLink
Log in

Exogenous 4-hydroxybenzoic acid and salicylic acid modulate the effect of short-term drought and freezing stress on wheat plants

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

Exogenous salicylic acid has been shown to confer tolerance against biotic and abiotic stresses. In the present work the ability of its analogue, 4-hydroxybenzoic acid to increase abiotic stress tolerance was demonstrated: it improved the drought tolerance of the winter wheat (Triticum aestivum L.) cv. Cheyenne and the freezing tolerance of the spring wheat cv. Chinese Spring. Salicylic acid, however, reduced the freezing tolerance of Cheyenne and the drought tolerance of Chinese Spring, in spite of an increase in the guaiacol peroxidase and ascorbate peroxidase activity. The induction of cross tolerance between drought and freezing stress was observed: drought acclimation increased the freezing tolerance of Cheyenne plants and cold acclimation enhanced the drought tolerance. The induction of drought tolerance in Cheyenne was correlated with an increase in catalase activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

APX:

ascorbate peroxidase

CAT:

catalase

Chl:

chlorophyll

Fm :

maximal fluorescence

Fv :

variable fluorescence

GPX:

guaiacol peroxidase

GR:

glutathione reductase

4-HBA:

4-hydroxybenzoic acid

PAL:

phenylalanine ammonialyase

PEG:

polyethylene glycol

PPFD:

photosynthetic photon flux density

PR:

pathogenesis-related

PS 2:

photosystem 2

SA:

salicylic acid

SAR:

systemic acquired resistance

References

  • Ádám, A., Bestwick, C.S., Barna, B., Mansfield, J.W.: Enzymes regulating the accumulation of active oxygen species during the hypersensitive reaction of bean to Pseudomonas syringae pv. phaseolica.-Planta 197: 240–249, 1995.

    Article  Google Scholar 

  • Agarwal, S., Sairam, R.K., Srivastava, R.K., Meena, R.C.: Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes.-Biol. Plant. 49: 541–550, 2005.

    Article  CAS  Google Scholar 

  • Ananieva, E.A., Christov, K.N., Popova, L.P.: Exogenous treatment with salicylic acid leads to increased antioxidant capacity in leaves of barley plants exposed to Paraquat.-J. Plant Physiol. 161: 319–328, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Bach, M., Schnitzler, J.P., Seitz, H.U.: Elicitor induced changes in Ca2+ influx, K+ efflux and 4-hydroxybenzoic acid synthesis in protoplasts of Daucus carota L.-Plant Physiol. 103: 407–412, 1993.

    PubMed  CAS  Google Scholar 

  • Baczek-Kwinta, R., Filek, W., Grzesiak, S., Hura, T.: The effect of soil drought and rehydration on growth and antioxidative activity in flag leaves of triticale.-Biol. Plant. 50: 55–60, 2006.

    Article  CAS  Google Scholar 

  • Baek, K.H., Skinner, D.Z.: Alteration of antioxidant enzyme gene expression during cold acclimation of near-isogenic wheat lines.-Plant Sci. 165: 1221–1227, 2003.

    Article  CAS  Google Scholar 

  • Borsani, O., Valpuesta, V., Botella, M.A.: Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis seedlings.-Plant Physiol. 126: 1024–1030, 2001.

    Article  PubMed  CAS  Google Scholar 

  • Bradford, M.M.: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248–254, 1976.

    Article  PubMed  CAS  Google Scholar 

  • Chen, Z., Ricigliano, J.R., Klessig, D.F.: Purification and characterization of a soluble salicylic acid binding protein from tobacco.-Proc. nat. Acad. Sci. USA 90: 9533–9537, 1993.

    Article  PubMed  CAS  Google Scholar 

  • Dat, J.F., Lopez-Delgado, H., Foyer, C.H., Scott, I.M.: Parallel changes in H2O2 and catalase during thermotolerance induced by salicylic acid or heat acclimation in mustard seedlings.-Plant Physiol. 116: 1351–1357, 1998.

    Article  CAS  PubMed  Google Scholar 

  • Duran-Serantes, B., Gonzalez, L., Reigosa, M.J.: Comparative physiological effects of three allelochemicals and two herbicides on Dactylis glomerata.-Acta Physiol. Plant. 24: 385–392, 2002.

    CAS  Google Scholar 

  • Hamada, A.M.: Effects of exogenously added ascorbic acid, thiamin or aspirin on photosynthesis and some related activities of drought-stressed wheat plants.-In: Garab, G. (ed.): Photosynthesis: Mechanisms and Effects. Vol. 4. Pp. 2581–2584. Kluwer Acad. Publ., Dordrecht 1998.

    Google Scholar 

  • Jain, M., Nandwal, A.S., Kundu, B.S., Kumar, B., Sheoran, I.S., Kumar, N., Mann, A., Kukreja, S.: Water relations, activities of antioxidants, ethylene evolution and membrane integrity of pigeonpea roots as affected by soil moisture.-Biol. Plant. 50: 303–306, 2006.

    Article  CAS  Google Scholar 

  • Janda, T.: Use of chlorophyll fluorescence induction techniques in the study of low temperature stress in plants.-Acta agron. hung. 46: 77–91, 1998.

    Google Scholar 

  • Janda, T., Szalai, G., Kissimon, J., Páldi, E., Marton, C., Szigeti, Z.: Role of light intensity in the chilling injury of young maize plants studied by chlorophyll fluorescence induction measurements.-Photosynthetica 30: 293–299, 1994.

    CAS  Google Scholar 

  • Janda, T., Szalai, G., Rios-Gonzalez, K., Veisz, O., Páldi, E.: Comparative study of frost tolerance and antioxidant activity in cereals.-Plant Sci. 164: 301–306, 2003.

    Article  CAS  Google Scholar 

  • Janda, T., Szalai, G., Tari, I., Páldi, E.: Hydroponic treatment with salicylic acid decreases the effect of chilling injury in maize (Zea mays L.) plants.-Planta 208: 175–180, 1999.

    Article  CAS  Google Scholar 

  • Klessig, D.F., Durner, J., Noad, R., Navarre, D.A., Wendehenne, D., Kumar, D., Zhou, J.M., Shah, J., Zhang, S., Kachroo, P., Trifa, Y., Pontier, D., Lam, E., Silva, H.: Nitric oxid and salicylic acid signalling in plant defense.-Proc. nat. Acad. Sci. USA 97: 8849–8855, 2000.

    Article  PubMed  CAS  Google Scholar 

  • Koch, W., Wagner, C., Seitz, H.U.: Elicitor-induced cell death and phytoalexin synthesis in Daucus carota L.-Planta 206: 523–532, 1998.

    Article  CAS  Google Scholar 

  • Kocsy, G., Galiba, G., Brunold, C.: Role of glutathione in adaptation and signalling during chilling and cold acclimation in plants.-Physiol. Plant. 113: 158–164, 2001.

    Article  PubMed  CAS  Google Scholar 

  • Lopez-Delgado, H., Dat, J.F., Foyer, C.H., Scott, I.M.: Induction of thermotolerance in potato microplants by acetylsalicylic acid and H2O2.-J. exp. Bot. 49: 713–720, 1998.

    Article  CAS  Google Scholar 

  • Moharekar, S.T., Lokhande, S.D., Hara, T., Tanaka, R., Tanaka, A., Chavan, P.D.: Effect of salicylic acid on chlorophyll and carotenoid contents of wheat and moong seedlings.-Photosynthetica 41: 315–317, 2003.

    Article  CAS  Google Scholar 

  • Molassiotis, A., Diamantidis, G., Therios, I., Dimassi, K.: Effects of salicylic acid on ethylene induction and antioxidant activity in peach rootstock regenerants.-Biol. Plant. 49: 609–612, 2005.

    Article  CAS  Google Scholar 

  • Munne-Bosch, S., Peñuelas, J.: Photo-and antioxidative protection, and a role for salicylic acid during drought and recovery in field-grown Phillyrea angustifolia plants.-Planta 217: 758–766, 2003.

    Article  PubMed  CAS  Google Scholar 

  • Nakano, Y., Asada, Y.: Purification of ascorbate peroxidase from spinach chloroplasts: its activation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical.-Plant Cell Physiol. 28: 131–140, 1987.

    CAS  Google Scholar 

  • Németh, M., Janda, T., Horváth, E., Páldi, E., Szalai, G.: Exogenous salicylic acid increases polyamine content but may decrease drought tolerance in maize.-Plant Sci. 162: 569–574, 2002.

    Article  Google Scholar 

  • Okuda, T., Matsuda, Y., Yamanaka, A., Sagisaka, S.: Abrupt increase in the level of hydrogen peroxide in leaves of wheat is caused by cold treatment.-Plant Physiol. 97: 1265–1267, 1991.

    Article  PubMed  CAS  Google Scholar 

  • Pál, M., Nagy, E.: Chilling tolerance of maize.-Acta agron. hung. 50: 95–106, 2002.

    Article  Google Scholar 

  • Rai, V.K., Sharma, S.S., Sharma, S.: Reversal of ABA-induced stomatal closure by phenolic compounds.-J. exp. Bot. 37: 129–134, 1986.

    Article  CAS  Google Scholar 

  • Rao, M.V., Paliyath, G., Ormrod, D.P., Murr, D.P., Watkins, C.B.: Influence of salicylic acid on H2O2 production, oxidative stress, and H2O2-metabolizing enzymes.-Plant Physiol. 115: 137–149, 1997.

    Article  PubMed  CAS  Google Scholar 

  • Senaratna, T., Touchell, D., Bunn, E., Dixon, K.: Acetyl salicylic acid (aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants.-Plant Growth Regul. 30: 157–161, 2000.

    Article  CAS  Google Scholar 

  • Shakirova, F.M., Sakhabutdinova, A.R., Bezrukova, M.V., Fatkhutdinova, R.A., Fatkhutdinova, D.R.: Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity.-Plant Sci. 164: 317–322, 2003.

    Article  CAS  Google Scholar 

  • Smith, I.K., Vierheller, T.L., Thorne, C.A.: Assay of glutathione reductase in crude tissue homogenates using 5,5′-dithiobis-(2-nitrobenzoic acid).-Anal. Biochem. 175: 408–413, 1988.

    Article  PubMed  CAS  Google Scholar 

  • Smith-Becker, J., Marois, E., Huguet, E.J., Midland, S.L., Sims, J.J., Keen, N.T.: Accumulation of salicylic acid and 4-hydroxybenzoic acid in phloem fluids of cucumber during systemic acquired resistance is preceded by a transient increase in phenylalanine ammonia-lyase in petioles and stems.-Plant Physiol. 116: 231–238, 1998.

    Article  PubMed  CAS  Google Scholar 

  • Szalai, G., Tari, I., Janda, T., Pestenácz, A., Páldi, E.: Effects of cold acclimation and salicylic acid on changes in ACC and MACC contents in maize during chilling.-Biol. Plant. 43: 637–640, 2000.

    Article  CAS  Google Scholar 

  • Tan, J.W., Bednarek, P., Liu, H.K., Schneider, B., Svatos, A., Hahlbrock, K. Universally occurring phenylpropanoid and species-specific indolic metabolites in infected and uninfected Arabidopsis thaliana roots and leaves.-Phytochemistry 65: 691–699, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Tasgín, E., Atící, Ö., Nalbantoglu, B.: Effects of salicylic acid and cold on freezing tolerance in winter wheat leaves.-Plant Growth Regul. 41: 231–236, 2003.

    Article  Google Scholar 

  • Thomashow, M.F.: So what’s new in the field of plant cold acclimation? Lots!-Plant Physiol. 125: 89–93, 2001.

    Article  PubMed  CAS  Google Scholar 

  • Ulmasov, T., Hagen, G., Guilfoyle, T.: The ocs element in the soybean GH2/4 promoter is activated by both active and inactive auxin and salicylic acid analogs.-Plant mol. Biol. 26: 1055–1064, 1994.

    Article  PubMed  CAS  Google Scholar 

  • Van der Straeten, D., Chaerle, L., Sharkov, G., Lambers, H., Van Montagu, M.: Salicylic acid enhances the activity of the alternative pathway of respiration in tobacco leaves and induces thermogenicity.-Planta 196: 412–419, 1995.

    Article  Google Scholar 

  • Willekens, H., Chamnongpol, S., Davey, M., Schaudner, M., Langebartels, C., Van Montagu, M., Inzé, D., Van Camp, W.: Catalase is a sink for H2O2 and is indispensible for stress defence in C3 plants.-EMBO J. 16: 4806–4816, 1997.

    Article  PubMed  CAS  Google Scholar 

  • Yu, X.-M., Griffith, M., Wiseman, S.B.: Ethylene induces antifreeze activity in winter rye leaves.-Plant Physiol. 126: 1232–1240, 2001.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Agricultural Research Institute of the Hungarian Academy of Sciences, H-2462, Martonvásár, POB 19, Hungary

    E. Horváth, M. Pál, G. Szalai, E. Páldi & T. Janda

Authors
  1. E. Horváth
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Pál
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Szalai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. Páldi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. Janda
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to T. Janda.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Horváth, E., Pál, M., Szalai, G. et al. Exogenous 4-hydroxybenzoic acid and salicylic acid modulate the effect of short-term drought and freezing stress on wheat plants. Biol Plant 51, 480–487 (2007). https://doi.org/10.1007/s10535-007-0101-1

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10535-007-0101-1

Additional key words

Search

Navigation