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Comparative proteomic analysis of two tobacco (Nicotiana tabacum) genotypes differing in Cd tolerance

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Abstract

Tobacco can easily accumulate cadmium (Cd) in leaves and thus poses a potential threat to human health. Cd-stress-hydroponic-experiments were performed, and the proteomic and transcriptional features of two contrasting tobacco genotypes Yun-yan2 (Cd-tolerant) and Guiyan1 (Cd-sensitive) were compared. We identified 18 Cd-tolerance-associated proteins in leaves, using 2-dimensional gel electrophoresis coupled with mass spectrometry, whose expression were significantly induced in Yunyan2 leaves but down-regulated/unchanged in Guiyan1, or unchanged in Yunyan2 but down-regulated in Guiyan1 under 50 µM Cd stress. They are including epoxide hydrolase, enoyl-acyl-carrier-protein reductase, NPALDP1, chlorophyll a–b binding protein 25, heat shock protein 70 and 14-3-3 proteins. They categorized as 8 groups of their functions: metabolism, photosynthesis, stress response, signal transduction, protein synthesis, protein processing, transport and cell structure. Furthermore, the expression patterns of three Cd-responsive proteins were validated by quantitative real-time PCR. Our findings provide an insight into proteomic basis for Cd-detoxification in tobacco which offers molecular resource for Cd-tolerance.

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Acknowledgments

We are thankful for the financial support from Guizhou Tobacco Science Institute.

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Authors and Affiliations

  1. Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Lupeng Xie, Xiaoyan He, Weite Zheng, Wenxing Liu, Guoping Zhang & Feibo Wu

  2. Guizhou Tobacco Science Institute, Guiyang, People’s Republic of China

    Shenghua Shang

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  1. Lupeng Xie
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  2. Xiaoyan He
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  4. Weite Zheng
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  7. Feibo Wu
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Correspondence to Feibo Wu.

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Xie, L., He, X., Shang, S. et al. Comparative proteomic analysis of two tobacco (Nicotiana tabacum) genotypes differing in Cd tolerance. Biometals 27, 1277–1289 (2014). https://doi.org/10.1007/s10534-014-9789-5

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