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Heme iron state in various oxyhemoglobins probed using Mössbauer spectroscopy with a high velocity resolution

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Abstract

A comparative study of oxyhemoglobins from pig, rabbit, normal human and patients with blood system malignant diseases was performed using Mössbauer spectroscopy with a high velocity resolution at 90 K. Mössbauer spectra were fitted with the help of two models: using one quadrupole doublet (model of equivalent iron electronic structure in α- and β-subunits of hemoglobins) and superposition of two quadrupole doublets (model of non-equivalent iron electronic structure in α- and β-subunits of hemoglobins). The results obtained using both models demonstrated small variations of hyperfine parameters that were related to the heme iron state variation in different hemoglobins. These results were compared with structural and functional differences of the hemoglobins investigated.

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Acknowledgments

This work was supported in part by the Russian Foundation for Basic Research (Grant # 09-02-00055-a) for M.I.O and Department of Biotechnology, Government of India (BT/PR11275/GBD/27/150/2008) for S.K. The financial assistance to A.K. in the form of a research fellowship by CSIR, Government of India is also duly acknowledged.

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Correspondence to M. I. Oshtrakh or S. Kundu.

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Oshtrakh, M.I., Berkovsky, A.L., Kumar, A. et al. Heme iron state in various oxyhemoglobins probed using Mössbauer spectroscopy with a high velocity resolution. Biometals 24, 501–512 (2011). https://doi.org/10.1007/s10534-011-9428-3

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  • DOI: https://doi.org/10.1007/s10534-011-9428-3

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