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Old carbon in young fine roots in boreal forests

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Abstract

A large proportion of the soil carbon (C) in boreal forests originates from roots and ectomycorrhizal fungi, and accurate quantification of fine-root litter production is needed. Methods for determination of root turnover have been under debate in recent years. Two recently used methods—radiocarbon (14C) dating and use of minirhizotrons (MR)—have yielded different results. This has been attributed to analysis of different roots by use of these methods. At Flakaliden, northern Sweden, in a long-term soil warming and irrigation experiment, we compared MR lifespan with the 14C-derived age of fine roots from soil cores of the same root diameter class. We also determined the 14C-derived age of ingrowth core roots of Norway spruce. The median lifespan of fine roots around MR tubes installed 15 years previously was shorter than 2.5 years whereas the 14C-derived age of the fine roots from soil cores varied from recently grown to 14 years. Correspondingly, the age of 14C in fine roots harvested from ingrowth cores installed in soil 3 months previously was between 1 and 20 years. Thus, cellulose in these roots contained older 14C than is possible from photosynthesis during the time of cellulose formation. By investigating whether the age of Norway spruce and Scots pine seedlings was less than their root 14C-derived age, we tested the possibility of root C originating from soil uptake. This was found to be unlikely, because fine roots of four and eight-year-old seedlings had 14C that was dated to be as old as or younger than the seedlings. We propose that further effort is required to identify the ecological conditions leading to root growth utilization of stored or recycled C.

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References

  • Adams TS, Eissenstat DM (2014) The continuous incorporation of carbon into existing sassafras albidum fine roots and its implications for estimating root turnover. PLoS One 9(5):e95321

    Article  Google Scholar 

  • Ahrens B, Hansson K, Solly EF, Schrumpf M (2014) Reconcilable differences: a joint calibration of fine-root turnover times with radiocarbon and minirhizotrons. New Phytol 204:932–942

    Article  Google Scholar 

  • Andersson P, Berggren D, Nilsson I (2002) Indices for nitrogen status and nitrate leaching from Norway spruce (Picea abies (L.) Karst.) stands in Sweden. For Ecol Manag 157:39–53

    Article  Google Scholar 

  • Berggren D, Bergkvist B, Johansson MB et al (2004) A description of LUSTRA’s common field sites. Rep For Ecol For Soils 87:1–42

    Google Scholar 

  • Bergh J, Linder S (1999) Effects of soil warming during spring on photosynthetic recovery in boreal Norway spruce stands. Glob Change Biol 5:245–253

    Article  Google Scholar 

  • Boutton TW, Wong WW, Hachey DL, Lee LS, Cabrera MP, Klein PD (1983) Comparison of quartz and pyrex tubes for combustion of organic samples for stable carbon isotope analysis. Anal Chem 55:1832–1833

    Article  Google Scholar 

  • Clemmensen KE, Bahr A, Ovaskainen O, Dahlberg A, Ekblad A, Wallander H, Stenlid J, Finlay RD, Wardle DA, Lindahl BD (2013) Roots and associated fungi drive long-term carbon sequestration in boreal forest. Science 339:1615–1618

    Article  Google Scholar 

  • Ford CR, Wurzburger N, Hendrick RL, Teskey RO (2007) Soil DIC uptake and fixation in Pinus taeda seedlings and its C contribution to plant tissues and ectomycorrhizal fungi. Tree Physiol 27:375–383

    Article  Google Scholar 

  • Freeman SPHT, Dougans A, McHargue L, Wilcken KM, Xu S (2008) Performance of the new single stage accelerator mass spectrometer at the SUERC. Nucl Instrum Methods 66:2225–2228

    Article  Google Scholar 

  • Fröberg M (2012) Residence time of fine-root carbon using radiocarbon measurements of samples collected from a soil archive. J Plant Nutr Soil Sci 175:46–48

    Article  Google Scholar 

  • Gaudinski JB, Trumbore SE, Davidson EA, Cook AC, Markewitz D, Richter DD (2001) The age of fine-root carbon in three forests of the eastern United States measured by radiocarbon. Oecologia 129(3):420–429

    Article  Google Scholar 

  • Gaudinski JB, Torn MS, Riley WJ, Swanston TS, Joslin JD, Majdi H, Dawson T, Hanson PJ (2009) Use of stored carbon reserves in growth of temperate tree roots and leaf buds: analyses using radiocarbon measurements and modeling. Glob Change Biol 15(4):992–1014

    Article  Google Scholar 

  • Gaudinski JB, Torn MS, Riley WJ, Dawson TE, Joslin JD, Majdi H (2010) Measuring and modeling the spectrum of fine-root turnover times in three forests using isotopes, minirhizotrons, and the Radix model. Glob Biogeochem Cycles 24:3029

    Article  Google Scholar 

  • Gaul D, Hertel D, Leuschner C (2009) Estimating fine root longevity in a temperate Norway spruce forest using three independent methods. Funct Plant Biol 36(1):11–19

    Article  Google Scholar 

  • Godbold DL, Fritz HW, Jentschke G, Meesenburg H, Rademacher P (2003) Root turnover and root necromass accumulation of Norway spruce (Picea abies) are affected by soil acidity. Tree Physiol 23:915–921

    Article  Google Scholar 

  • Guo DL, Li H, Mitchell RJ, Han W, Hendricks JJ, Fahey TJ, Hendrick RL (2008) Fine root heterogeneity by branch order: exploring the discrepancy in root turnover estimates between minirhizotron and carbon isotopic methods. New Phytol 172:523–535

    Google Scholar 

  • Hansson K, Helmisaari H-S, Sah S, Lange H (2013) Fine root production and turnover of tree and understorey vegetation in Scots pine, silver birch and Norway spruce stands in SW Sweden. For Ecol Manag 309:58–65

    Article  Google Scholar 

  • Helmisaari H, Ostonen I, Lõhmus K, Derome J, Lindroos A, Merila P, Nöjd P (2009) Ectomycorrhizal root tips in relation to site and stand characteristics in Norway spruce and Scots pine stands in boreal forests. Tree Physiol 29:445–456

    Article  Google Scholar 

  • Hendricks JJ, Hendrick RL, Wilson CA, Mitchell RJ, Pecot SD, Guo DL (2006) Assessing the patterns and controls of fine root dynamics: an empirical test and methodological review. J Ecol 94:40–57

    Article  Google Scholar 

  • Hobbie EA, Ouimette AP, Schuur EAG, Kierstead D, Trappe JM, Bendiksen K, Ohenoja E (2013) Radiocarbon evidence for the mining of organic nitrogen from soil by mycorrhizal fungi. Biogeochemistry 114:381–389

    Google Scholar 

  • Högberg P, Högberg MN, Gottlicher SG, Betson NR, Keel SG, Metcalfe DB, Campbell C, Schindlbacher A, Hurry V, Lundmark T, Linder S, Näsholm T (2008) High temporal resolution tracing of photosynthate carbon from the tree canopy to forest soil microorganisms. New Phytol 177(1):220–228

    Google Scholar 

  • Hua Q, Barbetti M (2004) Review of tropospheric bomb 14C data for carbon cycle modeling and age calibration purposes. Radiocarbon 46(3):1273–1298

    Google Scholar 

  • Hua Q, Barbetti M, Rakowski AZ (2013) Atmospheric radiocarbon for the period 1950–2010. Radiocarbon 55(4):2059–2072

    Article  Google Scholar 

  • Jones DL, Nguyen C, Finlay RD (2009) Carbon flow in the rhizosphere: carbon trading at the soil–root interface. Plant Soil 321(1–2):5–33

    Article  Google Scholar 

  • Joslin JD, Gaudinski JB, Torn MS, Riley WJ, Hanson PJ (2006) Fine-root turnover patterns and their relationship to root diameter and soil depth in a 14C-labeled hardwood forest. New Phytol 172(3):523–535

    Article  Google Scholar 

  • Keel S, Campbell CD, Högberg M, Richter A, Wild B, Zhou X, Hurry V, Linder S, Näsholm T, Högberg P (2012) Allocation of carbon to fine root compounds and their residence times in a boreal forest depend on root size class and season. New Phytol 194:972–981

    Article  Google Scholar 

  • Leppälammi-Kujansuu J, Berggren DK, Nilsson LO, Ostonen I, Sah SP, Helmisaari H-S (2013) Effects of long-term temperature and nutrient manipulation on Norway spruce fine roots and mycelia production. Plant Soil 366:287–303

    Article  Google Scholar 

  • Leppälammi-Kujansuu J, Salemaa M, Kleja DB, Linder S, Helmisaari H-S (2014a) Fine root turnover and litter production of Norway spruce in a long-term temperature and nutrient manipulation experiment. Plant Soil 374:73–88

    Article  Google Scholar 

  • Leppälammi-Kujansuu J, Aro L, Salemaa M, Hansson K, Berggren Kleja D, Helmisaari H-S (2014b) Fine root longevity and carbon input into soil from below- and aboveground litter in climatically contrasting forests. For Ecol Manag 326:79–90

    Article  Google Scholar 

  • Levin I, Kromer B (2004) The tropospheric 14CO2 level in mid latitudes of the Northern Hemisphere. Radiocarbon 46(3):1261–1272

    Google Scholar 

  • Levin I, Kromer B, Hammer S (2013) Atmospheric 14CO2 trend in Western European background air from 2000 to 2012. Tellus B 65:20092

    Article  Google Scholar 

  • Linder S (1995) Foliar analysis for detecting and correcting nutrient imbalances in Norway spruce. Ecol Bull (Copenhagen) 44:178–190

    Google Scholar 

  • McCormack ML, Dickie IA, Eissenstat DM, Fahey TJ, Fernandez CW, Guo D, Helmisaari H-S, Hobbie EA, Iversen CM, Jackson RB, Leppälammi-Kujansuu J, Norby RJ, Phillips RP, Pregitzer KS, Pritchard SG, Rewald B, Zadworny M (2015) Redefining fine roots improves understanding of belowground contributions to terrestrial biosphere processes. New Phytol. doi:10.1111/nph.13363)

    Google Scholar 

  • Näsholm T, Kielland K, Ganeteg U (2009) Uptake of organic nitrogen by plants. New Phytol 182:31–48

    Article  Google Scholar 

  • Ostonen I, Lõhmus K, Helmisaari H-S, Truu J, Meel S (2007) Fine root morphological adaptations in Scots pine, Norway spruce and silver birch along a latitudinal gradient in boreal forests. Tree Physiol 27:1627–1634

    Article  Google Scholar 

  • Pregitzer KS, DeForest JL, Burton AJ, Allen MF, Ruess RW, Hendrick RL (2002) Fine root architecture of nine North American trees. Ecol Monogr 72:293–309

    Article  Google Scholar 

  • Pritchard SG, Strand AE (2008) Can you believe what you see? Reconciling minirhizotron and isotopically derived estimates of fine root longevity. New Phytol 177:287–291

    Article  Google Scholar 

  • Reimer PJ, Bard E, Bayliss A, Beck JW, Blackwell PG, Bronk Ramsey C, Buck CE, Cheng H, Edwards RL, Friedrich M, Grootes PM, Guilderson TP, Haflidason H, Hajdas I, Hatte C, Heaton TJ, Hogg AG, Hughen KA, Kaiser KF, Kromer B, Manning SW, Niu M, Reimer RW, Richards DA, Scott EM, Southon JR, Turney CSM, van der Plicht J (2013) IntCal13 and MARINE13 radiocarbon age calibration curves 0-50000 years calBP. Radiocarbon 55(4):1869–1887

    Article  Google Scholar 

  • Sah SP, Jungner H, Oinonen M, Helmisaari H-S (2011) Does the age of fine root carbon indicate the age of fine roots in boreal forests? Biogeochemistry 104:91–102

    Article  Google Scholar 

  • Sah SP, Bryant C, Leppälammi-Kujansuu J, Lõhmus K, Ostonen I, Helmisaari H-S (2013) Variation of carbon age of fine roots in the boreal conifer forests determined from 14C measurements. Plant Soil 363:77–86

    Article  Google Scholar 

  • Scott EM (2003) The third international radiocarbon intercomparison (TIRI) and the fourth international radiocarbon intercomparison (FIRI), 1990–2002. Results, analyses, and conclusions. Radiocarbon 45(2):135–408

    Google Scholar 

  • Slota PJ, Jull AJT, Linick TW, Toolin LJ (1986) Preparation of small samples for 14C accelerator targets by catalytic reduction of CO. Radiocarbon 29(2):303–306

    Google Scholar 

  • Solly E, Schöning I, Boch S, Müller J, Socher SA, Trumbore SE, Schrumpf M (2013) Mean age of carbon in fine roots from temperate forests and grasslands with different management. Biogeosci Discuss 10:5671–5700

    Article  Google Scholar 

  • Strand AE, Pritchard SG, McCormack ML, Davis MA, Oren R (2008) Irreconcilable differences: fine root life spans and soil carbon persistence. Science 319(5862):456–458

    Article  Google Scholar 

  • Strömgren M, Linder S (2002) Effects of nutrition and soil warming on stemwood production in a boreal Norway spruce stand. Glob Change Biol 8:1194–1204

    Article  Google Scholar 

  • Stuiver M, Polach HA (1977) Discussion: reporting of 14C data. Radiocarbon 19(3):355–363

    Google Scholar 

  • Tierney GL, Fahey TJ (2002) Fine root turnover in a northern hardwood forest: a direct comparison of the radiocarbon and minirhizotron methods. Can J For Res 32(9):1692–1697

    Article  Google Scholar 

  • Vargas R, Trumbore SE, Allen MF (2009) Evidence of old carbon used to grow new fine roots in a tropical forest. New Phytol 182(3):710–718

    Article  Google Scholar 

  • Wells CE, Eissenstat DM (2001) Marked differences in survivorship among apple roots of different diameters. Ecology 82:882–893

    Article  Google Scholar 

  • Wells CE, Glenn DM, Eissenstat DM (2002) Changes in the risk of fine root mortality with age: a case study in peach, Prunus persica (Rosaceae). Am J Bot 89:79–87

    Article  Google Scholar 

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Acknowledgments

We are grateful to forest engineer Pekka Välikangas, Mr Juha Kemppainen and Ms Ulla Raatikainen from the Finnish Forest Research Institute, Salla Office, for assistance with field work and root sorting, and to Professor Sune Linder for providing the facilities for this study at Flakaliden. Michael Bailey kindly revised the English language. The study was funded by the Academy of Finland and the Maj and Tor Nessling Foundation.

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Authors and Affiliations

  1. Department of Forest Sciences, University of Helsinki, P.O. Box 27, 00014, Helsinki, Finland

    H.-S. Helmisaari, J. Leppälammi-Kujansuu & S. Sah

  2. NERC Radiocarbon Facility (East Kilbride), Scottish Enterprise Technology Park, Rankine Avenue, East Kilbride, G75 OQF, Scotland, UK

    C. Bryant

  3. Department of Soil and Environment, Swedish University of Agricultural Sciences, P.O. Box 7001, 75007, Uppsala, Sweden

    D. B. Kleja

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  1. H.-S. Helmisaari
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  2. J. Leppälammi-Kujansuu
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Correspondence to H.-S. Helmisaari.

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Responsible Editor: W. Troy.

Electronic supplementary material

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10533_2015_110_MOESM1_ESM.docx

Appendix 1. Supplementary information. 14C-contents and 14C-derived ages and sample data of soil core roots in the organic (O) and mineral (M) soil layers at Flakaliden in irrigated (I) and warmed-irrigated (WI) plots, ingrowth core roots, and seedlings roots. Supplementary material 1 (DOCX 32 kb)

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Helmisaari, HS., Leppälammi-Kujansuu, J., Sah, S. et al. Old carbon in young fine roots in boreal forests. Biogeochemistry 125, 37–46 (2015). https://doi.org/10.1007/s10533-015-0110-7

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