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Phanerochaete chrysosporium inoculation shapes the indigenous fungal communities during agricultural waste composting

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Abstract

Inoculation with exogenous white-rot fungi has been proven to be an efficient method to promote lignocellulose biodegradation during agricultural waste composting. Indigenous fungal communities, the most important organisms responsible for mineralization and decomposition of lignocellulosic materials in composts, can be affected by sample properties and other biotic factors. This research was conducted to determine the effects of the Phanerochaete chrysosporium inoculation on the indigenous fungal communities during agricultural waste composting. Fungal communities in samples with different inoculation regimes were investigated by sequencing and quantitative PCR. Results showed that P. chrysosporium inoculants produced significant negative effects on the indigenous fungal community abundance during the thermophilic stage. Samples inoculated during Phase II contained higher proportion of Acremonium chrysogenum and Galactomyces geotrichum, while those non-inoculated samples were dominated by Coprinopsis cinerea and Scytalidium thermophilum. Moreover, the indigenous fungal community abundance was significantly correlated with the C/N ratio, water soluble carbon and moisture content (P < 0.05). Redundancy analysis indicated that the most variation in distribution of indigenous fungal community structure was statistically explained by nitrate, C/N ratio, and moisture content, factors which solely explained 29.6 % (F = 30.316, P = 0.002), 25.6 % (F = 26.191, P = 0.002) and 10.0 % (F = 10.249, P = 0.002) of the variation in the indigenous fungal community structure, respectively.

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Acknowledgments

The authors sincerely thank Editors and anonymous reviewers for suggestions on improving the paper. This word was jointly supported by the National Natural Science Foundation of China (51378190, 51039001, 50808072, 51108423, 51108178, 51009063), the Natural Science Foundation of Zhejiang Province (Y5100234), the Research Fund for the Doctoral Program of Higher Education of China (20100161110012), the Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling Open Fund (SWTR-2012-07), Shanghai Tongji Gao Tingyao Environmental Science and Technology Development Foundation (STGEF), and the Environmental Science Foundation supported by Professor Renbin Yang of Hunan Agricultural University (14YB14).

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Authors and Affiliations

  1. College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, China

    Jiachao Zhang & Hongli Huang

  2. College of Environmental Science and Engineering, Hunan University, Changsha, 410082, China

    Guangming Zeng, Yaoning Chen, Jie Liang, Chang Zhang, Binbin Huang, Ming Chen & Yi Zhu

  3. Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha, 410082, China

    Guangming Zeng, Yaoning Chen, Jie Liang, Chang Zhang, Binbin Huang, Ming Chen & Yi Zhu

  4. Department of Environmental Sciences, Rutgers University, New Brunswick, NJ, 08901, USA

    Weimin Sun

  5. Environmental Resources and Soil Fertilizer Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Man Yu

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  1. Jiachao Zhang
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  2. Guangming Zeng
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  11. Yi Zhu
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Correspondence to Guangming Zeng or Yaoning Chen.

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Zhang, J., Zeng, G., Chen, Y. et al. Phanerochaete chrysosporium inoculation shapes the indigenous fungal communities during agricultural waste composting. Biodegradation 25, 669–680 (2014). https://doi.org/10.1007/s10532-014-9690-5

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  • DOI: https://doi.org/10.1007/s10532-014-9690-5

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