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Study the biocatalyzing effect and mechanism of cellulose acetate immobilized redox mediators technology (CE-RM) on nitrite denitrification

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Abstract

The biocatalyzing effect of a novel cellulose acetate immobilized redox mediators technology (CE-RM) on nitrite denitrification process was studied with anthraquinone, 1,8-dichloroanthraquinone, 1,5-dichloroanthraquinone and 1,4,5,8-tetrachloroanthraquinone. The results showed that the immobilized 1,4,5,8-tetrachloroanthraquinone presented the best biocatalyzed effect which increased nitrite denitrification rate to 2.3-fold with 12 mmol/L 1,4,5,8-tetrachloroanthraquinone. The unequal biocatalyzing effect was due to the quantity and position of –Cl substituent in anthraquinone-structure. Moreover, the nitrite denitrification rate was increased with the oxidation reduction potential (ORP) values becoming more negative during the biocatalyzing process. The stabilized ORP value with 12 mmol/L immobilized 1,4,5,8-tetrachloroanthraquinone were 81 mV lower than the control. At the same time, the more OH− was produced with the higher nitrite removal rate achieved in the nitrite denitrification process. In addition, a positive linear correlation was found between the nitrite removal reaction constants k [gNO2 −–N/(gVSS d)] and immobilized 1,4,5,8-tetrachloroanthraquinone concentration (C 1,4,5,8-tetrachloroanthraquinone), which was k = 1.8443 C 1,4,5,8-tetrachloroanthraquinone + 33.75(R 2 = 0.9411). The initial nitrite concentration of 179 mgNO2 −–N/L resulted in the maximum nitrite removal rate, which was 6.526[gNO2 −–N/(gVSS d)]. These results show that the application of cellulose acetate immobilized redox mediators (CE-RM) can be valuable for increasing nitrite denitrification rate.

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Abbreviations

ORP:

Oxidation reduction potential

CE-RM:

Cellulose acetate immobilized redox mediators

OD:

Optical density

Q:

Quinones

QH2 :

Hydroquinone

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Acknowledgments

This study was supported by Supporting Program for Hundred Outstanding Innovation Talents in Hebei Universities (II) (BR2-211), Hebei Natural Science Foundation for Distinguished Young Scholars (Grant No. E2012208012) and Program for New Century Excellent Talents in University (Grant No. NCET-10-0127).

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Authors and Affiliations

  1. School of Environmental Science and Engineering, Hebei University of Science and Technology, Yuhua East Road 70#, Shijiazhuang, 050018, People’s Republic of China

    Haibo Li, Jianbo Guo, Jing Lian, Zhenhua Xi, Lijun Zhao, Xiaoyu Liu, Chenxiao Zhang & Jingliang Yang

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  1. Haibo Li
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  2. Jianbo Guo
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  3. Jing Lian
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  4. Zhenhua Xi
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  6. Xiaoyu Liu
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  7. Chenxiao Zhang
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Correspondence to Jianbo Guo.

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Li, H., Guo, J., Lian, J. et al. Study the biocatalyzing effect and mechanism of cellulose acetate immobilized redox mediators technology (CE-RM) on nitrite denitrification. Biodegradation 25, 395–404 (2014). https://doi.org/10.1007/s10532-013-9668-8

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