Skip to main content
SpringerLink
Log in

Spartina versicolor Fabre: Another case of Spartina trans-Atlantic introduction?

  • Invasive Spartina
  • Published:
Biological Invasions Aims and scope Submit manuscript

Abstract

Intercontinental introductions are widespread in the genus Spartina, with important ecological and evolutionary consequences. The native or introduced status of Spartina species is then critical with regard to biodiversity assessment, especially for vulnerable Mediterranean coastline ecosystems. Spartina versicolor was first recorded in southern France in 1849, then successively in various places on the European and North-African Mediterranean and Atlantic coasts. This species is considered to be either a European native or an invasive species introduced from North America which has a high morphological similarity to the Atlantic American species Spartina patens. We performed extensive sampling of S. versicolor in Europe and North Africa (from natural populations and herbarium collections) and compared these samples to other European and American Spartina species (including S. patens). Chromosome counts were reported for the first time and revealed that S. versicolor is tetraploid (2n = 4x = 40). Phylogenetic analyses based on chloroplast and nuclear ribosomal DNA sequences did not reveal any molecular variation within S. versicolor. In this species, a single haplotype, that is identical to one haplotype of S. patens, was found in the four chloroplast and the nuclear ribosomal ITS regions investigated. In addition, simple sequence repeat markers were used and revealed a low level of genetic diversity within S. versicolor, suggesting that the introduction of S. versicolor occurred from a narrow genetic pool of S. patens from North America.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Ainouche ML, Fortune PM, Salmon A, Parisod C, Grandbastien MA, Fukunaga K, Ricou M, Misset MT (2009) Hybridization, polyploidy and invasion: lessons from Spartina (Poaceae). Biol Invasions 11:1159–1173

  • Ainouche ML, Chelaifa H, Ferreira de Carvalho J, Bellot S, Ainouche AK, Salmon A (2012) Polyploid evolution in Spartina: dealing with highly redundant hybrid genomes. In: Soltis PS, Soltis DE (eds) Polyploidy and genome evolution. Springer, Berlin, pp 225–243

  • An SQ, Gu BH, Zhou CF et al (2007) Spartina invasion in China: implications for invasive species management and future research. Weed Res 47:183–191

    Article  Google Scholar 

  • Ayres DR, Smith DL, Zaremba K, Klohr S, Strong DR (2004) Spread of exotic cordgrass and hybrids (Spartina sp) in the tidal marshes of San-Francisco Bay CA, USA. Biol Invasions 6:221–231

    Article  Google Scholar 

  • Baumel A, Ainouche ML, Levasseur JE (2001) Molecular investigations in populations of Spartina anglica CE Hubbard (Poaceae) invading coastal Brittany (France). Mol Ecol 10:1689–1701

    Article  CAS  PubMed  Google Scholar 

  • Baumel A, Ainouche ML, Bayer RJ, Ainouche AK, Misset MT (2002) Molecular phylogeny of hybridizing species from the genus Spartina Schreb. (Poaceae). Mol Phylogenet Evol 22:303–314

    Article  CAS  PubMed  Google Scholar 

  • Baumel A, Ainouche ML, Misset MT, Gourret JP, Bayer RJ (2003) Genetic evidence for hybridization between the native Spartina maritima and the introduced Spartina alterniflora (Poaceae) in South-West France: Spartina neyrautii re-examined. Plant Syst Evol 237:87–97

    Article  CAS  Google Scholar 

  • Bertacci A, Lombardi T (2014) Spartina versicolor Fabre in coastal areas of Tuscany (Italy). Contrib Bot XLIX:49–60

    Google Scholar 

  • Blum MJ, Bando KJ, Katz M (2007) Geographic structure, genetic diversity and source tracking of Spartina alterniflora. J Biogeogr 34:2055–2069

    Article  Google Scholar 

  • Bortolus A (2006) The austral cordgrass Spartina densiflora Brong: its taxonomy, biogeography and natural history. J Biogeogr 33:158–168

    Article  Google Scholar 

  • Bortolus A, Carlton JT, Schwindt E (2015) Reimagining South American coasts: unveiling the hidden invasion history of an iconic ecological engineer. Divers Distrib 21:1267–1283

    Article  Google Scholar 

  • Boutte J, Aliaga B, Lima O, Ferreira de Carvalho J, Ainouche A, Macas J, Rousseau-Gueutin M, Coriton O, Ainouche M, Salmon A (2015) Haplotype detection from next-generation sequencing in high-ploidy-level species: 45S rDNA gene copies in the hexaploid spartina maritima. G3 6:29–40

    Article  PubMed  PubMed Central  Google Scholar 

  • Burdick DM, Mendelssohn IA (1987) Waterlogging responses in dune, swale, and marsh populations of Spartina patens under field conditions. Oecologia 74:321–329

    Article  Google Scholar 

  • Burdick DM, Mendelssohn IA, McKee KL (1989) Live standing crop and metabolism of the salt marsh grass Spartina patens as related to edaphic factors in a brackish, mixed marsh community in Louisiana. Estuaries 12:195–204

    Article  Google Scholar 

  • Campos JA, Herrera M, Biurrun I, Loidi J (2004) The role of alien plants in the natural coastal vegetation in central-northern Spain. Biodivers Conserv 13:2275–2293

    Article  Google Scholar 

  • Casolo V, Tomasella M, De Col V, Braidot E, Savi T, Nardini A (2014) Water relations of an invasive halophyte (Spartina patens): osmoregulation and ionic effects on xylem hydraulics. Funct Plant Biol 42:264–273

    Google Scholar 

  • Castillo JM, Mateos-Naranjo E, Nieva FJ, Figueroa E (2008) Plant zonation at salt marshes of the endangered cordgrass Spartina maritima invaded by Spartina densiflora. Hydrobiologia 614:363–371

    Article  Google Scholar 

  • Castillo JM, Ayres DR, Leira-Docel P, Bailey J, Blum M, Strong DR, Luque T, Figueroa E (2010) The production of hybrids with high ecological amplitude between exotic Spartina densiflora and native S maritima in the Iberian Peninsula. Divers Distrib 16:547–558

    Article  Google Scholar 

  • Chevalier MA (1923) Note sur les Spartina de la flore française. Bull Soc Bot Fr 70:54–63

    Article  Google Scholar 

  • Cosson E, Durieu de Maisonneuve (1867) Introduction à la flore d’Algérie. Phanérogamie. Groupe des Glumacées (seu descriptio glumacearum in Algeria nascentium). Exploration Scientifique de l’Algérie, publiée par ordre du Gouvernement. Sciences Naturelles. Botanique. Imprimerie Impériale. Paris

  • Coste H (1906) Flore de la France. T.III. Librairie des Sciences Naturelles, Paris, p 807

    Google Scholar 

  • Cottet M, De Montaudouin X, Blanchet H, Lebleu P (2007) Spartina anglica eradication experiment and in situ monitoring assess structuting strength of habitat complexity on marine macrofauna at high tidal level. Estuar Coast Shelf S 71:629–640

    Article  Google Scholar 

  • Daehler CC, Strong DR (1996a) Status, prediction and prevention of introduced cordgrass Spartina spp. invasions in Pacific estuaries, USA. Biol Conserv 78:51–58

    Article  Google Scholar 

  • Daehler CC, Strong DR (1996b) Status, prediction and prevention of introduced cordgrass Spartina spp. invasions in Pacific estuaries, USA. Biol Conserv 78:51–58

    Article  Google Scholar 

  • Daveau J (1897) La flore littorale du Portugal. Bol Soc Brot 14:4–54

    Google Scholar 

  • Dray S, Dufour AB (2007) The ade4 package: implementing the duality diagram for ecologists. J Stat Softw 22:1–20

    Article  Google Scholar 

  • Drummond AJ, Ashton B, Buxton S et al (2010) Geneious v5.1. http://www.geneious.com

  • Fabre ME (1849) Description d’une nouvelle espèce de Spartina, abondante sur une portion du littoral méditerranéen. Ann Sci Nat Bot Biol 3:122–125

    Google Scholar 

  • Felsenstein J (1985) Confidence-limits on phylogenies—an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Ferreira de Carvalho J, Chelaifa H, Boutte J et al (2013) Exploring the genome of the salt- marsh Spartina maritima (Poaceae, Chloridoideae) through BAC end sequence analysis. Plant Mol Biol 83:591–606

    Article  CAS  PubMed  Google Scholar 

  • Foote AL, Reynolds KA (1997) Decomposition of saltmeadow cordgrass (Spartina patens) in Louisiana coastal marshes. Estuaries 20:579–588

    Article  Google Scholar 

  • Fortune PM, Schierenbeck KA, Ainouche AK, Jacquemin J, Wendel JF et al (2007) Evolutionary dynamics of Waxy and the origin of hexaploid Spartina species (Poaceae). Mol Phylogenet Evol 43:1040–1055

    Article  CAS  PubMed  Google Scholar 

  • Fortune PM, Schierenbeck K, Ayres D, Bortolus A, Clatrice O, Ainouche ML (2008) The enigmatic invasive Spartina densiflora: a history of hybridizations in a polyploidy context. Mol Ecol 17:4304–4316

    Article  CAS  PubMed  Google Scholar 

  • Frasco BA, Good RE (1982) Decomposition dynamics of Spartina alterniflora and Spartina patens in a New Jersey salt marsh. Am J Bot 69:402–406

    Article  Google Scholar 

  • Giuliano M, Stanisci A (2010) Biodiversity conservation in coastal areas in molise (Italy). Bol Mus Ist Biol Univ Genova 72:144–147

    Google Scholar 

  • Gray AJ, Benham PEM, Raybould AF (1990) Spartina anglica—the evolutionary and ecological background. In: Gray AJ, Benham PEM (eds) Spartina anglica—a research review. Institute of Terrestrial Ecology, Natural Environment Research Council, 5–10

  • Gray AJ, Marshall DF, Raybould AF (1991) A century of evolution in Spartina anglica. Adv Ecol Res 21:1–62

    Article  Google Scholar 

  • Guénégou MC, Citharel J, Levasseur JE (1988) The hybrid status of Spartina anglica (Poaceae). Enzymatic analysis of the species and the presumed parents. Can J Bot 66:1830–1833

    Google Scholar 

  • Guindon S, Gascuel O (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704

    Article  PubMed  Google Scholar 

  • Hacker SD, Heimer D, Hellquist CE, Reeder TG, Reeves B, Riordan TJ Jr, Dethier MN (2001) A marine plant (Spartina anglica) invades widely varying habitats: potential mechanisms of invasion and control. Biol Invasions 3:211–217

    Article  Google Scholar 

  • Hardion L, Baumel A, Verlaque R, Vila B (2014) Distinct evolutionary histories of lowland biota on Italian and Balkan peninsulas revealed by the phylogeography of Arundo plinii (Poaceae). J Biogeogr 41:2150–2161

    Article  Google Scholar 

  • Hounsome G (2013) Spartina patens in West Sussex. Bot Soc Br Isles BBSI News 123:66–67

    Google Scholar 

  • Hubbard JCE (1968) Grasses, 2nd edn. Penguin Books, London

    Google Scholar 

  • Jaccard P (1901) Distribution de la flore alpine dans le bassin des Dranses et dans quelques régions voisines. Bull Soc Vaud Sci Nat 37:241–272

    Google Scholar 

  • Jeanmonod D, Burdet HM (1989) Notes et contributions à la flore de Corse IV. Candollea 44:337–401

    Google Scholar 

  • Jombart T (2008) Adegenet: a R package for the multivariate analysis of genetic markers. Bioinformatics 24:1403–1405

    Article  CAS  PubMed  Google Scholar 

  • Jombart T, Devillard S, Balloux F (2010) Discriminant analysis of principal components: a new method for the analysis of genetically structured populations. BMC Genet 11:94

    Article  PubMed  PubMed Central  Google Scholar 

  • Kim S, Rayburn AL, Voigt TB, AinoucheML Ainouche AK, Lee DK (2013) Chloroplast DNA intraspecific phylogeography of Prairie Cordgrass (Spartina pectinata Bosc ex Link). Plant Mol Biol Rep 31:1376–1383

    Article  Google Scholar 

  • Kowarik I (2003) Human agency in biological invasions: secondary releases foster naturalisation and population expansion of alien plant species. Biol Invasions 5:293–312

    Google Scholar 

  • Lonard RI, Judd FW, Stalter R (2010) The biological flora of coastal dunes and wetlands: Spartina patens (W. Aiton) G.H. Muhlenberg. J Coast Res 265:935–946

    Article  Google Scholar 

  • Mansion G, Rosenbaum G, Schoenenberger N, Bacchetta G, Rossell’o J, Conti E (2008) Phylogenetic analysis informed by geological history supports multiple, sequential invasions of the mediterranean basin by the angiosperm family Araceae. Syst Biol 57:269–285

    Article  CAS  PubMed  Google Scholar 

  • Marchant CJ (1968) Evolution in Spartina (Gramineae). III. Species chromosome numbers and their taxonomic significance. Bot J Linn Soc 60:41–47

    Google Scholar 

  • Marignani M, Bacchetta G, Bagella S, Carmela Caria M, Delogu F, Farris E, Fenu G, Filigheddu R, Blasi C (2014) Is time on our side? Strengthening the link between field efforts and conservation needs. Biodivers Conserv 23:421–431

    Article  Google Scholar 

  • Médail F, Verlaque R (1997) Ecological characteristics and rarity of endemic plants from southeast France and Corsica: implications for biodiversity conservation. Biol Conserv 80:269–281

    Article  Google Scholar 

  • Migliore J, Baumel A, Juin M, Fady B, Roig A, Duong N, Médail F (2013) Surviving in mountain climate refugia: new insights from the genetic diversity and structure of the relict shrub Myrtus nivellei (Myrtaceae) in the Sahara Desert. PLoS One 8:e73795

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mobberley D (1956) Taxonomy and distribution of the genus Spartina. Iowa State Coll J Sci 30:471–574

    Google Scholar 

  • Nei M (1972) Genetic distance between populations. Am Nat 106:283–292

    Article  Google Scholar 

  • Oksanen J, Blanchet FG, Kindt R et al (2013) vegan: community ecology package. R package version 2.0-7. http://CRAN.R-project.org/package=vegan

  • Page HM, Lastra M, Rodil IF et al (2010) Effects of non-native Spartina patens on plant and sediment organic matter carbon incorporation into the local invertebrate community. Biol Invasions 12:3825–3838

    Article  Google Scholar 

  • Paradis E, Claude J, Strimmer K (2004) APE: analyses of phylogenetics and evolution in R language. Bioinformatics 20:289–290

    Article  CAS  PubMed  Google Scholar 

  • Parlatore F (1850) Flora Italiana. I. Tipografia Le Monnier, Firenze

    Google Scholar 

  • Peakall R, Smouse PE (2006) GenAlEx 6: genetic analysis in excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295

    Article  Google Scholar 

  • Peterson PM, Romaschenko K, Herrera Arrieta Y, Saarela JM (2014) A molecular phylogeny and new subgeneric classification of Sporobolus (Poaceae: Chloridoideae: Sporobolinae). Taxon 63:1212–1243

    Article  Google Scholar 

  • Posada D (2008) jModelTest: phylogenetic model averaging. Mol Biol Evol 25:1253–1256

    Article  CAS  PubMed  Google Scholar 

  • Prieto JAF, Cires E, Sanchez Corominas T et al (2011) Systematics and management of natural resources: the case of Spartina species on European shores. Biologia 66:1011–1018

    Article  Google Scholar 

  • Rousseau-Gueutin M, Bellot S, Martin GE et al (2015) The chloroplast genome of the hexaploid Spartina maritima (Poaceae, Chloridoideae): comparative analyses and molecular dating. Mol Phylogenet Evol 93:5–16

    Article  CAS  PubMed  Google Scholar 

  • Saarela JM (2012) Taxonomic synopsis of invasive and native Spartina (Poaceae, Chloridoideae) in the Pacific Northwest (British Columbia, Washington and Oregon), including the first report of Spartina x townsendii for British Columbia, Canada. PhytoKeys 10:25–82

  • Saint-Yves A (1932) Monographia spartinum. Candollea 5:19–100

    Google Scholar 

  • Sanchéz-Gullón E (2001) Spartina versicolor (Poaceae): novedad agrotológica para Andalucía. Acta Liotan Malacit 26:279–280

    Google Scholar 

  • SanLeón DG, Izco J, Sánchez JM (1999) Spartina patens as a weed in Galician saltmarshes (NW Iberian Peninsula). Hydrobiologia 415:213–222

    Article  Google Scholar 

  • Sanz Elorza M, Dana Sánchez ED, Sobrino Vesperinas E (2004) Atlas de las plantas alóctonas invasoras en España. Dirección General para la Biodiversidad, Madrid, p 384

    Google Scholar 

  • Silander JA (1984) The genetic basis of the ecological amplitude of Spartina patens. III. Allozyme variation. Bot Gaz 569–577

  • Silander J, Antonovics J (1979) The genetic basis of the ecological amplitude of Spartina patens. Morphometric and physiological traits. Evolution 33:1114–1127

    Article  Google Scholar 

  • Stace C (2010) New flora of the British Isles. Cambridge University Press, Cambridge

    Google Scholar 

  • Strong DR, Ayres DR (2013) Ecological and evolutionary misadventures of Spartina. Ann Rev Ecol Evol Syst 44:389–410

    Article  Google Scholar 

  • Swofford DL (2001) Phylogenetic analysis using Parsimony (* and other methods). Version 4.0. Sinauer Associates. Sunderland, Massachusetts

  • Taberlet P, Gielly L, Pautou G, Bouvet J (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol Biol 17:1105–1109

  • Tamura K, Peterson D, Peterson N et al (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tison JM, de Foucault B (2014) Flora gallica. Flore de France, Biotope, Mèze

    Google Scholar 

  • Tison JM, Jauzein P, Michaud H (2014) Flore de la France Mediterranéenne Continentale. Naturalia, Porquerolles

    Google Scholar 

  • Valsecchi F (1962) Spartina juncea Willd. nuova specie per la Sardegna. Giorn Bot Italy 69:3–47

    Google Scholar 

  • Valtuena FJ, Preston CD, Kadereit JW (2011) Evolutionary significance of the invasion of introduced populations into the native range of Meconopsis cambrica. Mol Ecol 20:4318–4331

    Article  PubMed  Google Scholar 

  • Verlaque R, Aboucaya A, Fridlender A (2002) Les xénophytes envahissants en France: écologie, types biologiques et polyploïdie. Bot Helv 112:121–136

    Google Scholar 

  • White TJ, Bruns T, Lee S et al (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, New York, pp 315–322

    Google Scholar 

  • Wu T (2012) Genetic diversity in Spartina patens in remnant patches in the New Jersey Meadowlands. Master thesis. Rutgers University, New Jersey (USA). http://dx.doi.org/doi:10.7282/T39K498K

Download references

Acknowledgments

This work was supported by the “Biological Invasion” Programme of UMR-CNRS Ecobio, University of Rennes 1 (France), the IMBE molecular biology service, the International Associated Laboratory LIA ECOGEN and the Partner University Funds (to M. A.). We gratefully acknowledge financial support from the “Région Bretagne” and the European Union Seventh Framework Programme [FP7-CIG-2013–2017; Grant no. 333709 to M. R-G.]. We thank members of herbaria in France (Herbier Montpellier Université, Herbier du Muséum d’Histoire Naturelle de Marseille, Herbier Paris, Université de Rennes 1) for providing access to historical reference samples, and to the many people who helped us getting plant samples from various places (listed in Supplementary table S1), and most particularly: G. Martin, O. Garsmeur, E. Vela, J-M. Lewin, C. Piazza, J. Xiao Wen Wong, (Old World S. versicolor) M-E. Siqueiros, M. Gross and C. Richards (New World S. patens). The authors also thank D. Ayres and one anonymous reviewer for helpful comments on an earlier version of this manuscript.

Author information

Authors and Affiliations

  1. Aix Marseille Université, Institut Méditerranéen de Biodiversité et d’Ecologie (IMBE, UMR CNRS, IRD, Avignon Université), Technopôle de l’Environnement Arbois-Méditerranée, BP 80, 13545, Aix-en-Provence Cedex 04, France

    A. Baumel & N. Duong

  2. UMR CNRS 6553 Ecobio, OSUR (Observatoire des Sciences de l’Univers de Rennes), Université de Rennes 1/Université Européenne de Bretagne, 35042, Rennes, France

    M. Rousseau-Gueutin, C. Sapienza-Bianchi, A. Gareil, H. Rousseau & M. Ainouche

  3. INRA, UMR 1349, Institut de Génétique, Environnement et Protection des Plantes, 35653, Le Rheu Cedex, France

    M. Rousseau-Gueutin

  4. Plate-Forme de Cytogénétique Moléculaire, INRA, UMR 1349, Institut de Génétique, Environnement et Protection des Plantes, 35653, Le Rheu Cedex, France

    O. Coriton

  5. Université des Sciences et de la Technologie Houari Boumediene, Laboratoire de Biologie et Physiologie des Organismes, BP 32 El-Alia, 16111, Bab-Ezzouar, Alger, Algeria

    R. Amirouche

  6. Department of Biological, Geological and Environmental Sciences, University of Catania, via Alongo 18, 95125, Catania, Italy

    S. Sciandrello

  7. MARE – Marine and Environmental Sciences Centre, Faculty of Sciences of the University of Lisbon, Campo Grande, 1749-016, Lisbon, Portugal

    B. Duarte & I. Caçador

  8. Departamento de Biología Vegetal y Ecología, Facultad de Biologia, Universidad de Sevilla, Apartado 1095, 41080, Seville, Spain

    J. M. Castillo

Authors
  1. A. Baumel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Rousseau-Gueutin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Sapienza-Bianchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Gareil
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. N. Duong
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. H. Rousseau
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. O. Coriton
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. R. Amirouche
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. S. Sciandrello
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. B. Duarte
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. I. Caçador
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. J. M. Castillo
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. M. Ainouche
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Ainouche.

Additional information

Guest editors: Alan Gray and Malika Ainouche/Invasive Spartina.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (XLSX 18 kb)

Supplementary material 2 (XLSX 44 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Baumel, A., Rousseau-Gueutin, M., Sapienza-Bianchi, C. et al. Spartina versicolor Fabre: Another case of Spartina trans-Atlantic introduction?. Biol Invasions 18, 2123–2135 (2016). https://doi.org/10.1007/s10530-016-1128-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10530-016-1128-z

Keywords

Search

Navigation