Abstract
Hybridization associated with species introductions can accelerate the decline of native species. The main objective of this study was to determine if the decline of a North American liana (American bittersweet, Celastrus scandens) in the eastern portion of its range is related to hybridization with an introduced congener (oriental bittersweet, C. orbiculatus). We used newly characterized microsatellite loci, a maternally-inherited chloroplast DNA marker, and field observation to survey individuals across the USA to determine the prevalence of hybrids, their importance in the invasion of C. orbiculatus, and the predominant direction of hybridization. We found that only 8.4 % of non-native genotypes were hybrids (20 of 239), and these hybrids were geographically widespread. Hybrids showed reduced seed set (decline of >98 %) and small, likely inviable pollen. Genetic analysis of a maternally inherited chloroplast marker showed that all 20 identified hybrids came from C. scandens seed parents. The strong asymmetry in pollen flow that favors fecundity in introduced males has the potential to greatly accelerate the decline of native species by wasting limited female reproductive effort.
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Acknowledgments
We would like to thank Ralph Grundel and Krystal Frohnapple for assistance in planning and executing this study. Boris Igic, Jeremie Fant, Henry F. Howe, John Wilk, Emi Kuroiwa, Janet Backs, Eun Sun Kim, Jason Palagi, Wendy Stott, and Gina Morgan provided helpful comments that improved the manuscript. Sample collection was aided by Thomas Rawinksi, Ron Lance, John Balaban, Bob Stoos, and numerous National Park Service scientists. We would like to thank the National Park Service, Department of Environment and Conservation in Tennessee, DNR of Illinois, Indiana, and Michigan, North Carolina State Parks, and Forest Preserves Districts of Cook, Lake, and DuPage Counties in Illinois for permission to sample on their properties,. Microscopy and pollen grain measurement was aided by Jack Gibbons and Eun Sun Kim. Claudia Wing aided in development of the RFLP analysis. Genetic analysis was aided by Kirsten Dittel and Iryna Shak. Financial support was provided by the University of Illinois at Chicago, United States Geological Survey, and Chicago Wilderness. This article is contribution No. 1939 of the USGS Great Lakes Science Center. Use of trade, product, or firm names does not imply endorsement by the US Government. This manuscript was completed in partial fulfillment of the doctoral degree from the Graduate College at the University of Illinois at Chicago to D. N. Zaya.
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Zaya, D.N., Leicht-Young, S.A., Pavlovic, N.B. et al. Genetic characterization of hybridization between native and invasive bittersweet vines (Celastrus spp.). Biol Invasions 17, 2975–2988 (2015). https://doi.org/10.1007/s10530-015-0926-z
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DOI: https://doi.org/10.1007/s10530-015-0926-z