Abstract
Objective
To characterize transcriptome-wide lincRNAs of Hela-S3 cell line by analyzing RNA sequencing data to provide a foundation for further functional verification and clinical application of cervical carcinoma development.
Results
Single-cell RNA sequencing data of 37 Hela-S3 cells were analysed. On average, 511 lincRNAs were expressed in each cell. Comparing the expression difference of the lincRNAs and protein-coding genes, we found that lincRNAs expression displayed more cell specificity than that of protein-coding genes (t-test, P<2.2E-16). In co-expression network analysis, we identified seven modules and one of them was enriched in pathways of mitotic, packaging of telomere ends, and chromosome maintenance.
Conclusion
incRNAs are specifically expressed and form a network to perform function at single cell level. Their expression was more specific than that of protein-coding genes.
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Acknowledgements
We are grateful to Bo Li (BGI), Shiping Liu (BGI), Lei Xing (BGI) and Yan Wang (BISU) for a careful modification of the manuscript.
Supporting information
Supplementary Table 1—Summary of RNA-seq data and alignment.
Supplementary Table 2—The matrix of lincRNAs expression quantity in 37 Hela-S3 single cells.
Supplementary Table 3—The information of co-expression modules about 323 lincRNAs.
Supplementary Table 4—The GO and pathway annotation of co-expression modules.
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Wang, J., Roy, B. Single-cell RNA-seq reveals lincRNA expression differences in Hela-S3 cells. Biotechnol Lett 39, 359–366 (2017). https://doi.org/10.1007/s10529-016-2260-7
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DOI: https://doi.org/10.1007/s10529-016-2260-7