Abstract
Objectives
To design and construct a novel bio-anode electrode based on the oxidation of glutamic acid to produce 2-oxoglutarate, generating two electrons from NADH.
Results
Efficient enzyme reaction and electron transfer were observed owing to immobilization of the two enzymes using a mixed self-assembled monolayer. The ratio of the immobilized enzymes was an important factor affecting the efficiency of the system; thus, we quantified the amounts of immobilized enzyme using a quartz crystal microbalance to further evaluate the electrochemical reaction. The electrochemical reaction proceeded efficiently when approximately equimolar amounts of the enzyme were on the electrode. The largest oxidation peak current increase (171 nA) was observed under these conditions.
Conclusion
Efficient multi-enzyme reaction on the electrode surface has been achieved which is applicable for biofuel cell application.
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Supporting information
Supplementary Table 1—Purification table of proline dehydrogenase.
Supplementary Table 2—Purification table of Pis-glutamate dehydrogenase.
Supplementary Fig. 1—SDS-PAGE of purified proline dehydrogenase.
Supplementary Fig. 2—SDS-PAGE of purified Pis-glutamate dehydrogenase.
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Hiroaki Sakamoto has contributed equally to this work.
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Sakamoto, H., Komatsu, T., Yamasaki, K. et al. Design of a multi-enzyme reaction on an electrode surface for an l-glutamate biofuel anode. Biotechnol Lett 39, 235–240 (2017). https://doi.org/10.1007/s10529-016-2237-6
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DOI: https://doi.org/10.1007/s10529-016-2237-6