Abstract
Objective
To increase the production of 10S-hydroxy-8(E)-octadecenoic acid from oleic acid by whole recombinant Escherichia coli cells expressing Nostoc punctiforme 10S-dioxygenase with the aid of a chaperone.
Results
The optimal conditions for 10S-hydroxy-8(E)-octadecenoic acid production by recombinant cells co-expressing chaperone plasmid were pH 9, 35 °C, 15 % (v/v) dimethyl sulfoxide, 40 g cells l−1, and 10 g oleic acid l−1. Under these conditions, recombinant cells co-expressing chaperone plasmid produced 7.2 g 10S-hydroxy-8(E)-octadecenoic acid l−1 within 30 min, with a conversion yield of 72 % (w/w) and a volumetric productivity of 14.4 g l−1 h−1.
Conclusion
The activity of recombinant cells expressing 10S-dioxygenase was increased by 200 % with the aid of a chaperone, demonstrating the first biotechnological production of 10S-hydroxy-8(E)-octadecenoic acid using recombinant cells expressing 10S-dioxygenase.
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Acknowledgments
This study was supported by a grant from the Korea Healthcare Technology R&D Project, Ministry for Health & Welfare, Republic of Korea (No. 2012-009).
Supporting information
Supplementary Fig. 1—LC–MS/MS spectra for the products obtained from the conversion of unsaturated fatty acids to 10S-hydroxy fatty acids by recombinant cells expressing 10S-dioxygenase from N. punctiforme PCC 73102.
Supplementary Fig. 2—Effects of pH and temperature on the conversion of oleic acid to 10S-hydroxy-8(E)-octadecenoic acid by recombinant cells co-expressing the 10S-dioxygenase gene of N. punctiforme with pGro7.
Supplementary Fig. 3—Effect of dimethyl sulfoxide concentration on the conversion of oleic acid to 10S-hydroxy-8(E)-octadecenoic acid by recombinant cells co-expressing the 10S-dioxygenase gene of N. punctiforme with pGro7.
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Kim, MJ., Seo, MJ., Shin, KC. et al. Production of 10S-hydroxy-8(E)-octadecenoic acid from oleic acid by whole recombinant Escherichia coli cells expressing 10S-dioxygenase from Nostoc punctiforme PCC 73102 with the aid of a chaperone. Biotechnol Lett 39, 133–139 (2017). https://doi.org/10.1007/s10529-016-2225-x
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DOI: https://doi.org/10.1007/s10529-016-2225-x