Abstract
Objective
To determine whether the G–H loop of foot-and-mouth disease virus (FMDV) serotype O can function as a target structure to harbour and display serotype Asia1 antigenic epitope at the surface.
Results
Using reverse genetics, FMDV serotype O IND R2/1975 displaying a FMDV serotype Asia1 B cell epitope at the capsid surface was constructed. The epitope-inserted recombinant chimeric virus was genetically stable up to ten serial passages in cell culture and exhibited growth properties similar to the parental serotype O virus. Furthermore, the surface-displayed Asia1 epitope able to react with serotype Asia1 specific antibodies in a competitive ELISA. Importantly, the recombinant chimeric virus showed neutralizing activity to both serotype O and Asia1 polyclonal antibodies.
Conclusion
The capsid protein of FMDV serotype O can effectively display potent epitope of other serotypes, making this an attractive approach for the design of new generation bi-valent FMD vaccines.
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Acknowledgments
This work was supported by Indian Council of Agricultural Research (ICAR) under the Project IXX10081.
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Biswal, J.K., Ranjan, R. & Pattnaik, B. Chimeric foot-and-mouth disease virus serotype O displaying a serotype Asia1 antigenic epitope at the surface. Biotechnol Lett 38, 1509–1517 (2016). https://doi.org/10.1007/s10529-016-2121-4
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DOI: https://doi.org/10.1007/s10529-016-2121-4