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Long-term response on growth, antioxidant enzymes, and secondary metabolites in salicylic acid pre-treated Uncaria tomentosa microplants

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Abstract

Objective

To obtain micro propagated Uncaria tomentosa plantlets with enhanced secondary metabolites production, long-term responses to salicylic acid (SA) pre-treatments at 1 and 100 µM were evaluated after propagation of the plantlets in a SA-free medium.

Results

SA pre-treatments of single node cuttings OF U. tomentosa produced long-term responses in microplants grown for 75 days in a SA-free medium. Reduction in survival rate, root formation, and stem elongation were observed only with 100 µM SA pre-treatments with respect to the control (0 + DMSO).Both pre-treatments enhanced H2O2 and inhibited superoxide dismutase and catalase activities, while guaiacol peroxidase was increased only with 1 µM SA. Also, both pre-treatments increased total monoterpenoid oxindole alkaloids by ca. 55 % (16.5 mg g−1 DW), including isopteropodine, speciophylline, mitraphylline, isomitraphylline, rhynchopylline, and isorhynchopylline; and flavonoids by ca. 21 % (914 μg g−1 DW), whereas phenolic compounds were increased 80 % (599 μg g−1 DW) at 1 µM and 8.2 % (359 μg g−1 DW) at 100 µM SA.

Conclusion

Pre-treatment with 1 µM SA of U.tomentosa microplants preserved the survival rate and increased oxindole alkaloids, flavonoids, and phenolic compounds in correlation with H2O2 and peroxidase activity enhancements, offering biotechnological advantages over non-treated microplants.

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Acknowledgments

This work was supported by Consejo Nacional de Ciencia y Tecnología (Conacyt-Mexico, grants 105019 and 222097). Silvia Sánchez-Rojo acknowledges Conacyt-Mexico for a doctoral fellowship (211538). Authors wish to thank M Sc Gabriela Luna-Palencia and Dr Ileana Vera-Reyes for advices in chromatographic analyses and Carmen Fontaine for technical support.

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Authors and Affiliations

  1. Departamento de Biotecnología y Bioingeniería, Centro de Investigación y deEstudios Avanzados del Instituto Politécnico Nacional, Av. I. P. N. 2508. Col. San Pedro Zacatenco, 07360, Mexico, D.F., Mexico

    Silvia Sánchez-Rojo, Fernando Esparza-García, Héctor M. Poggi-Varaldo, Teresa Ponce-Noyola & Ana C. Ramos-Valdivia

  2. Departamento de Química, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av. I. P. N. 2508. Col. San Pedro Zacatenco, 07360, Mexico, D.F., Mexico

    Carlos M. Cerda-García-Rojas

  3. Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, 04510, Mexico, D.F., Mexico

    Javier Plasencia

Authors
  1. Silvia Sánchez-Rojo
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  2. Carlos M. Cerda-García-Rojas
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  3. Fernando Esparza-García
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  6. Teresa Ponce-Noyola
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  7. Ana C. Ramos-Valdivia
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Correspondence to Ana C. Ramos-Valdivia.

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Sánchez-Rojo, S., Cerda-García-Rojas, C.M., Esparza-García, F. et al. Long-term response on growth, antioxidant enzymes, and secondary metabolites in salicylic acid pre-treated Uncaria tomentosa microplants. Biotechnol Lett 37, 2489–2496 (2015). https://doi.org/10.1007/s10529-015-1931-0

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  • DOI: https://doi.org/10.1007/s10529-015-1931-0

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