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Production of conjugated linoleic acid by heterologous expression of linoleic acid isomerase in oleaginous fungus Mortierella alpina

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Abstract

Objective

To increase the commercial value of oleaginous fungus Mortierella alpina by incorporation of trans-10,cis-12 conjugated linoleic acid (CLA) into the polyunsaturated fatty acids (PUFAs) of M. alpina via Propionibacterium acnes isomerase (PAI) conversion.

Results

The PAI gene and the codon optimization version were heterologously expressed in M. alpina via Agrobacterium tumefaciens-mediated transformation (ATMT). Coding usage modification significantly improved the translation of PAI transcripts and trans-10,cis-12 CLA was produced up to 1.2 mg l−1, which corresponds to approx. 0.05 % of the total fatty acid (TFA). Since PAI prefers free linoleic acid as a substrate rather than any other forms, 5 μM long-chain acyl CoA synthetase inhibitor was added and the trans-10,cis-12 CLA content increased approx. 24-fold to 29 mg l−1, reaching up to 1.2 % (w/w) of the TFA in M. alpina.

Conclusion

Heterologous expression of PAI in M. alpina by ATMT methods is a practicable way in biosynthesis of CLA and this system may be a feasible platform for industrial production of CLA.

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Acknowledgments

This study was supported by the Program for New Century Excellent Talents (NCET-13-0831), the National Natural Science Foundation of China (Nos. 21276108, 31125021, 31471128, 31400038), the Program for Changjiang Scholars and Innovative Research Team in University (IRT1249), and the Fundamental Research Funds for the Central Universities (No. JUSRP51320B).

Conflict of interest

There are no conflicts of interest.

Supporting information

Supplementary Table 1—Primers used in this study.

Supplementary Table 2—Dry cell weight and accumulation of lipid in M. alpine transformant with addition of ACSL inhibitor.

Author information

Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, People’s Republic of China

    Danhui Hao, Haiqin Chen, Guangfei Hao, Bo Yang, Baixi Zhang, Hao Zhang, Wei Chen & Yong Q. Chen

  2. Synergistic Innovation Center for Food Safety and Nutrition, Wuxi, 214122, People’s Republic of China

    Haiqin Chen, Guangfei Hao, Bo Yang, Baixi Zhang, Hao Zhang, Wei Chen & Yong Q. Chen

  3. Departments of Cancer Biology and Biochemistry, Wake Forest School of Medicine, Winston-Salem, NC, 27157, USA

    Yong Q. Chen

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  1. Danhui Hao
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  2. Haiqin Chen
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  3. Guangfei Hao
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  4. Bo Yang
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  6. Hao Zhang
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  7. Wei Chen
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Corresponding author

Correspondence to Haiqin Chen.

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Hao, D., Chen, H., Hao, G. et al. Production of conjugated linoleic acid by heterologous expression of linoleic acid isomerase in oleaginous fungus Mortierella alpina . Biotechnol Lett 37, 1983–1992 (2015). https://doi.org/10.1007/s10529-015-1871-8

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  • DOI: https://doi.org/10.1007/s10529-015-1871-8

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