Abstract
Objectives
To obtain a novel adaptable stain that can produce enantioselective l-lactic acid efficiently, reduce the operational cost of fermentation process and be suitable for scale production.
Results
Enterococcus faecium S.156 produced 126 g l-lactic acid/l with high optical purity (99.7 %), high productivity (5.25 g/l.h) and a conversion ratio >90 %. l-lactic acid production remained steady from 32 to 40 °C and at pH values from 5.5 to 6.5. O2 made no difference to both biomass growth and the l-lactic acid fermentation. 8 mg folic acid/l combined with 2 mM Fe2+ substituted for 6 g yeast extract/l, which is a cost-saving.
Conclusions
The special characteristics and economic traits of E. faecium S.156 make it suitable for industrial-scale production of l-lactic acid.
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Acknowledgments
This work was supported by a grant from Taishan Scholar Construction Project of Shandong Province.
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Sun, W., Liu, J., Xu, H. et al. l-Lactic acid fermentation by Enterococcus faecium: a new isolate from bovine rumen. Biotechnol Lett 37, 1379–1383 (2015). https://doi.org/10.1007/s10529-015-1821-5
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DOI: https://doi.org/10.1007/s10529-015-1821-5