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Deletion of d-ribulose-5-phosphate 3-epimerase (RPE1) induces simultaneous utilization of xylose and glucose in xylose-utilizing Saccharomyces cerevisiae

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Abstract

Simultaneous co-utilization of xylose and glucose is a key issue in engineering microbes for cellulosic ethanol production. We coupled xylose utilization with glucose metabolism by deletion of d-ribulose-5-phosphate 3-epimerase (RPE1) through pentose phosphate pathway flux. Simultaneous utilization of xylose and glucose then occurred in the engineered Saccharomyces cerevisiae strain with the xylose utilization pathway. Xylose consumption occurred at the beginning of glucose consumption by the engineered yeast without RPE1 in a mixed sugar fermentation. About 3.2 g xylose l−1 was utilized simultaneously with consumption of 40.2 g glucose l−1 under O2-limited conditions. In addition, an approximate ratio (~1:10) for xylose and glucose consumption was observed in the fermentation with different sugar concentration by the engineered strain without RPE1. Simultaneous utilization of xylose is realized by the coupling of glucose metabolism and xylose utilization through RPE1 deletion in xylose-utilizing S. cerevisiae.

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Acknowledgments

This work was funded by the National Natural Science Foundation of China (21390203) and Ministry of Science and Technology of China (“973” Program: 2013CB733600).

Supporting information

Supplementary Table 1 – Primers used in the study.

Supplementary Figure 1 - Fermentation performance of SyBE_Sc17002 in medium containing 40 g glucose l−1 and 10 g xylose l-1. The initial cell density was adjusted to OD600 = 1.

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Authors and Affiliations

  1. Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, People’s Republic of China

    Ming-Hua Shen, Hao Song, Bing-Zhi Li & Ying-Jin Yuan

  2. SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

    Ming-Hua Shen, Hao Song, Bing-Zhi Li & Ying-Jin Yuan

Authors
  1. Ming-Hua Shen
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  2. Hao Song
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  3. Bing-Zhi Li
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Correspondence to Bing-Zhi Li.

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Shen, MH., Song, H., Li, BZ. et al. Deletion of d-ribulose-5-phosphate 3-epimerase (RPE1) induces simultaneous utilization of xylose and glucose in xylose-utilizing Saccharomyces cerevisiae . Biotechnol Lett 37, 1031–1036 (2015). https://doi.org/10.1007/s10529-014-1759-z

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  • DOI: https://doi.org/10.1007/s10529-014-1759-z

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