Abstract
Cytosolic ascorbate peroxidase 1 (APX1) plays a crucial role in regulating the level of plant cellular reactive oxygen species and its thermolability is proposed to cause plant heat-susceptibility. Herein, several hyper-acidic fusion partners, such as the C-terminal peptide tails, were evaluated for their effects on the thermal stability and activity of APX1 from Jatropha curcas and Arabidopsis. The hyper-acidic fusion partners efficiently improved the thermostability and prevented thermal inactivation of APX1 in both plant species with an elevated heat tolerance of at least 2 °C. These hyper-acidified thermostable APX1 fusion variants are of considerable biotechnological potential and can provide a new route to enhance the heat tolerance of plant species especially of inherent thermo-sensitivity.
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Acknowledgments
This work was supported by several grants from the National Foundations of Natural Sciences, China (No. 31260064 to M. Gong, No. 31060160, 31160169 to Z. Zou).
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Zhang, M., Gong, M., Yang, Y. et al. Improvement on the thermal stability and activity of plant cytosolic ascorbate peroxidase 1 by tailing hyper-acidic fusion partners. Biotechnol Lett 37, 891–898 (2015). https://doi.org/10.1007/s10529-014-1754-4
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DOI: https://doi.org/10.1007/s10529-014-1754-4