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An investigation into the stability of commercial versus MG63-derived hepatocyte growth factor under flow cultivation conditions

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Abstract

The scale-up of tissue engineering cell culture must ensure that conditions are maintained while also being cost effective. Here we analyse the stability of hepatocyte growth factor (HGF) to investigate whether concentrations change under dynamic conditions, and compare commercial recombinant human HGF as an additive in ‘standard medium’, to HGF secreted by the osteosarcoma cell line MG63 as a ‘preconditioned medium’. After 3 h under flow conditions, HGF in the standard medium degraded to 40 % of its original concentration but HGF in the preconditioned medium remained at 100 %. The concentration of secreted HGF was 10 times greater than the working concentration of commercially-available HGF. Thus HGF within this medium has increased stability; MG63-derived HGF should therefore be investigated as a cost-effective alternative to current lyophilised powders for use in in vitro models. Furthermore, we recommend that those intending to use HGF (or other growth factors) should consider similar stability testing before embarking on experiments with media flow.

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Acknowledgments

This work was supported by a Marie Curie Early Stage Training Fellowship award (Meneghello). The authors would like to thank Dr Alexander Ciupa for his technical assistance.

Conflict of interest

No conflicts of interest exist for any of the authors.

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Authors and Affiliations

  1. Department of Pharmacy and Pharmacology, Centre for Regenerative Medicine, University of Bath, Bath, BA2 7AY, UK

    Giulia Meneghello & Paul A. De Bank

  2. Department of Chemical Engineering, Centre for Regenerative Medicine, University of Bath, Bath, BA2 7AY, UK

    Giulia Meneghello, Michael P. Storm, Julian B. Chaudhuri & Marianne J. Ellis

  3. Department of Neuroscience, Janssen Research and Development, Janssen Pharmaceutica, Turnhoutseweg 30, 2340, Beerse, Belgium

    Giulia Meneghello

  4. School of Engineering and Informatics, University of Bradford, Bradford, BD7 1DP, UK

    Julian B. Chaudhuri

Authors
  1. Giulia Meneghello
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  2. Michael P. Storm
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  3. Julian B. Chaudhuri
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  4. Paul A. De Bank
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  5. Marianne J. Ellis
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Correspondence to Marianne J. Ellis.

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Meneghello, G., Storm, M.P., Chaudhuri, J.B. et al. An investigation into the stability of commercial versus MG63-derived hepatocyte growth factor under flow cultivation conditions. Biotechnol Lett 37, 725–731 (2015). https://doi.org/10.1007/s10529-014-1701-4

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  • DOI: https://doi.org/10.1007/s10529-014-1701-4

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