Abstract
Spin-filters have been primarily used for producing therapeutic proteins from mammalian cells. However, disposability and/or high filter clogging of the existing spin-filter systems affect the process economy and productivity. Hence, to address these drawbacks a reusable dual spin-filter module for perfusion culture of adherent and non-adherent mammalian cells was designed. Two non-woven Bombyx mori silk layers were used as filter screen; the outer layer was conducive to cell attachment whilst the inner was non-conducive. Adherent cells can be cultured either in suspended mode using its inner single module or as monolayer of cells using its dual concentric module. We achieved 30 % higher urokinase productivity as compared to the stainless-steel spin-filter during perfusion experiments of adherent human kidney cells in suspended mode. This was due to the hydrophobic and negatively-charged silk screen that allows clog-free perfusion culture for prolonged periods.
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The authors gratefully acknowledge the funding received for this work from the Department of Biotechnology, Ministry of Science and Technology, Government of India.
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Kamthan, S., Gomes, J. & Roychoudhury, P.K. Silk screen based dual spin-filter module for perfusion culture of adherent and non-adherent mammalian cells. Biotechnol Lett 36, 1623–1630 (2014). https://doi.org/10.1007/s10529-014-1524-3
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DOI: https://doi.org/10.1007/s10529-014-1524-3