Abstract
The synthesis of monodispersed magnetic silica nanoparticles (MSN) is described using a water-in-oil reverse microemulsion system that does not require the use of co-surfactants. Sodium silicate, Tween 20 as a neutral surfactant and 1-butanol as the organic phase were used. There are several advantages of the proposed method including a saturation magnetization value of 10 emu/g for the particles obtained, uniformity of size and that they are easily functionalized to bind urease covalently. Moreover, the intra-day, inter-day and long-term stability results confirm that the procedure was successful and the enzyme-linked MSNs were stable over repeated uses and storage retaining more than 75 % activity after 4 months.
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Acknowledgments
G.S.A is grateful for her postdoctoral fellowship granted by the National Research Council (CONICET). The authors would like to acknowledge the support of grants from the University of Buenos Aires UBACYT B049 (to L.E.D.) and 20020090200051 (to M.F.D.) and from Agencia Nacional de Investigaciones Científicas y Técnicas BID 1728/OC-AR PICT 2008-1783 and PICT 2012-1441.
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Tuttolomondo, M.V., Villanueva, M.E., Alvarez, G.S. et al. Preparation of submicrometer monodispersed magnetic silica particles using a novel water in oil microemulsion: properties and application for enzyme immobilization. Biotechnol Lett 35, 1571–1577 (2013). https://doi.org/10.1007/s10529-013-1259-6
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DOI: https://doi.org/10.1007/s10529-013-1259-6