Original Research Paper

Biotechnology Letters

, Volume 35, Issue 10, pp 1631-1637

Metabolic engineering of Escherichia coli for enhanced biosynthesis of poly(3-hydroxybutyrate) based on proteome analysis

  • Seung Hwan LeeAffiliated withIndustrial Biochemicals Research Group, Division of Convergence Chemistry, Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology
  • , Kyoung-Hee KangAffiliated withIndustrial Biochemicals Research Group, Division of Convergence Chemistry, Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology
  • , Eun Young KimAffiliated withIndustrial Biochemicals Research Group, Division of Convergence Chemistry, Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology
  • , Tong Un ChaeAffiliated withMetabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST
  • , Young Hoon OhAffiliated withIndustrial Biochemicals Research Group, Division of Convergence Chemistry, Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology
  • , Soon Ho HongAffiliated withSchool of Chemical Engineering and Bioengineering, University of Ulsan
  • , Bong Keun SongAffiliated withIndustrial Biochemicals Research Group, Division of Convergence Chemistry, Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology
  • , Jonggeon JegalsAffiliated withIndustrial Biochemicals Research Group, Division of Convergence Chemistry, Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology
  • , Si Jae ParkAffiliated withDepartment of Environmental Engineering and Energy, Department of Energy and Biotechnology, Myongji University Email author 
    • , Sang Yup LeeAffiliated withMetabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAISTDepartment of Bio and Brain Engineering, Department of Biological Sciences, BioProcess Engineering Research Center, and Bioinformatics Research Center, KAIST Email author 

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Abstract

We have previously analyzed the proteome of recombinant Escherichia coli producing poly(3-hydroxybutyrate) [P(3HB)] and revealed that the expression level of several enzymes in central metabolism are proportional to the amount of P(3HB) accumulated in the cells. Based on these results, the amplification effects of triosephosphate isomerase (TpiA) and fructose-bisphosphate aldolase (FbaA) on P(3HB) synthesis were examined in recombinant E. coli W3110, XL1-Blue, and W lacI mutant strains using glucose, sucrose and xylose as carbon sources. Amplification of TpiA and FbaA significantly increased the P(3HB) contents and concentrations in the three E. coli strains. TpiA amplification in E. coli XL1-Blue lacI increased P(3HB) from 0.4 to 1.6 to g/l from glucose. Thus amplification of glycolytic pathway enzymes is a good strategy for efficient production of P(3HB) by allowing increased glycolytic pathway flux to make more acetyl-CoA available for P(3HB) biosynthesis.

Keywords

FbaA TpiA Poly(3-hydroxybutyrate) Proteome analysis Recombinant E. coli