Biotechnology Letters

, Volume 35, Issue 10, pp 1631–1637

Metabolic engineering of Escherichia coli for enhanced biosynthesis of poly(3-hydroxybutyrate) based on proteome analysis

Authors

  • Seung Hwan Lee
    • Industrial Biochemicals Research Group, Division of Convergence ChemistryResearch Center for Biobased Chemistry, Korea Research Institute of Chemical Technology
  • Kyoung-Hee Kang
    • Industrial Biochemicals Research Group, Division of Convergence ChemistryResearch Center for Biobased Chemistry, Korea Research Institute of Chemical Technology
  • Eun Young Kim
    • Industrial Biochemicals Research Group, Division of Convergence ChemistryResearch Center for Biobased Chemistry, Korea Research Institute of Chemical Technology
  • Tong Un Chae
    • Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program)Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST
  • Young Hoon Oh
    • Industrial Biochemicals Research Group, Division of Convergence ChemistryResearch Center for Biobased Chemistry, Korea Research Institute of Chemical Technology
  • Soon Ho Hong
    • School of Chemical Engineering and Bioengineering, University of Ulsan
  • Bong Keun Song
    • Industrial Biochemicals Research Group, Division of Convergence ChemistryResearch Center for Biobased Chemistry, Korea Research Institute of Chemical Technology
  • Jonggeon Jegals
    • Industrial Biochemicals Research Group, Division of Convergence ChemistryResearch Center for Biobased Chemistry, Korea Research Institute of Chemical Technology
    • Department of Environmental Engineering and Energy, Department of Energy and BiotechnologyMyongji University
    • Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program)Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST
    • Department of Bio and Brain Engineering, Department of Biological SciencesBioProcess Engineering Research Center, and Bioinformatics Research Center, KAIST
Original Research Paper

DOI: 10.1007/s10529-013-1246-y

Cite this article as:
Lee, S.H., Kang, K., Kim, E.Y. et al. Biotechnol Lett (2013) 35: 1631. doi:10.1007/s10529-013-1246-y

Abstract

We have previously analyzed the proteome of recombinant Escherichia coli producing poly(3-hydroxybutyrate) [P(3HB)] and revealed that the expression level of several enzymes in central metabolism are proportional to the amount of P(3HB) accumulated in the cells. Based on these results, the amplification effects of triosephosphate isomerase (TpiA) and fructose-bisphosphate aldolase (FbaA) on P(3HB) synthesis were examined in recombinant E. coli W3110, XL1-Blue, and W lacI mutant strains using glucose, sucrose and xylose as carbon sources. Amplification of TpiA and FbaA significantly increased the P(3HB) contents and concentrations in the three E. coli strains. TpiA amplification in E. coli XL1-Blue lacI increased P(3HB) from 0.4 to 1.6 to g/l from glucose. Thus amplification of glycolytic pathway enzymes is a good strategy for efficient production of P(3HB) by allowing increased glycolytic pathway flux to make more acetyl-CoA available for P(3HB) biosynthesis.

Keywords

FbaATpiAPoly(3-hydroxybutyrate)Proteome analysisRecombinant E. coli

Supplementary material

10529_2013_1246_MOESM1_ESM.docx (24 kb)
Supplementary material 1 (DOCX 23 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2013