Biotechnology Letters

, Volume 35, Issue 5, pp 685–688

Enantioselective synthesis of (S)-2-cyano-2-methylpentanoic acid by nitrilase

  • Toyokazu Yoshida
  • Koichi Mitsukura
  • Takuya Mizutani
  • Ryo Nakashima
  • Yasuyo Shimizu
  • Hiroshi Kawabata
  • Toru Nagasawa
Original Research Paper

DOI: 10.1007/s10529-012-1131-0

Cite this article as:
Yoshida, T., Mitsukura, K., Mizutani, T. et al. Biotechnol Lett (2013) 35: 685. doi:10.1007/s10529-012-1131-0

Abstract

The nitrilase gene of Rhodococcus rhodochrous J1 was expressed in Escherichia coli using the expression vector, pKK223-3. The recombinant E. coli JM109 cells hydrolyzed enantioselectively 2-methyl-2-propylmalononitrile to form (S)-2-cyano-2-methylpentanoic acid (CMPA) with 96 % e.e. Under optimized conditions, 80 g (S)-CMPA l−1 was produced with a molar yield of 97 % at 30 °C after a 24 h without any by-products.

Keywords

NitrilaseMalononitrileEnantioselective hydrolysisRhodococcus

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Toyokazu Yoshida
    • 1
  • Koichi Mitsukura
    • 1
  • Takuya Mizutani
    • 1
  • Ryo Nakashima
    • 1
  • Yasuyo Shimizu
    • 1
  • Hiroshi Kawabata
    • 2
  • Toru Nagasawa
    • 1
  1. 1.Department of Biomolecular ScienceGifu UniversityGifuJapan
  2. 2.Biotechnology Laboratory, Mitsubishi Chemical Group Science and Technology Research Center, Inc.YokohamaJapan