Biotechnology Letters

, Volume 35, Issue 5, pp 799–810

Mapping of the quantitative trait locus (QTL) conferring partial resistance to rice leaf blast disease

  • S. Ashkani
  • M. Y. Rafii
  • H. A. Rahim
  • M. A. Latif
Original Research Paper

DOI: 10.1007/s10529-012-1130-1

Cite this article as:
Ashkani, S., Rafii, M.Y., Rahim, H.A. et al. Biotechnol Lett (2013) 35: 799. doi:10.1007/s10529-012-1130-1

Abstract

Malaysian rice, Pongsu Seribu 2, has wide-spectrum resistance against blast disease. Chromosomal locations conferring quantitative resistance were detected by linkage mapping with SSRs and quantitative trait locus (QTL) analysis. For the mapping population, 188 F3 families were derived from a cross between the susceptible cultivar, Mahsuri, and a resistant variety, Pongsu Seribu 2. Partial resistance to leaf blast in the mapping population was assessed. A linkage map covering ten chromosomes and consisting of 63 SSR markers was constructed. 13 QTLs, including 6 putative and 7 putative QTLs, were detected on chromosomes 1, 2, 3, 5, 6, 10, 11 and 12. The resulting phenotypic variation due to a single QTL ranged from 2 to 13 %. These QTLs accounted for approx. 80 % of the total phenotypic variation within the F3 population. Therefore, partial resistance to blast in Pongsu Seribu 2 is due to combined effects of multiple loci with major and minor effects.

Keywords

Leaf blastPartial resistanceQuantitative trait locus (QTL)Rice (Oryza sativa)SSR markers

Supplementary material

10529_2012_1130_MOESM1_ESM.doc (70 kb)
Supplementary material 1 (DOC 70 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • S. Ashkani
    • 1
    • 2
  • M. Y. Rafii
    • 1
    • 3
  • H. A. Rahim
    • 4
  • M. A. Latif
    • 1
    • 5
  1. 1.Department of Crop Science, Faculty of AgricultureUniversiti Putra MalaysiaSerdangMalaysia
  2. 2.Department of Agronomy and Plant BreedingShahr-e-Rey Branch, Islamic Azad UniversityTehranIran
  3. 3.Institute of Tropical AgricultureUniversiti Putra MalaysiaSerdangMalaysia
  4. 4.Agrotechnology and Bioscience DivisionMalaysian Nuclear AgencyKajangMalaysia
  5. 5.Bangladesh Rice Research Institute (BRRI)GazipurBangladesh