Original Research Paper

Biotechnology Letters

, Volume 33, Issue 9, pp 1805-1808

Mechanism of rate enhancement of wood fiber saccharification by cationic polyelectrolytes

  • Sandeep MoraAffiliated withInstitute of Paper Science and Technology, School of Chemical & Biomolecular Engineering, Georgia Institute of Technology
  • , Jian LuAffiliated withInstitute of Paper Science and Technology, School of Chemical & Biomolecular Engineering, Georgia Institute of Technology
  • , Sujit BanerjeeAffiliated withInstitute of Paper Science and Technology, School of Chemical & Biomolecular Engineering, Georgia Institute of Technology Email author 

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Abstract

Cationic polyelectrolytes can increase the cellulase-induced hydrolysis rates of bleached wood fiber. We show that the polymer associates mainly with the amorphous region of fiber and acts principally on endoglucanase. Fiber/water partitioning of the enzyme follows a Langmuir isotherm for the untreated fiber but a Freundlich isotherm is obeyed for the polymer-treated fiber.

Keywords

Cationic polyacrylamide Cellulase Cellulose Endoglucanase