Biotechnology Letters

, Volume 32, Issue 5, pp 701–705

Regioselective carboxylation of catechol by 3,4-dihydroxybenzoate decarboxylase of Enterobacter cloacae P

  • Toyokazu Yoshida
  • Yuki Inami
  • Tsuyoshi Matsui
  • Toru Nagasawa
Original Research Paper

DOI: 10.1007/s10529-010-0210-3

Cite this article as:
Yoshida, T., Inami, Y., Matsui, T. et al. Biotechnol Lett (2010) 32: 701. doi:10.1007/s10529-010-0210-3

Abstract

3,4-Dihydroxybenzoate decarboxylase in Enterobacter cloacae P241 was induced by adding 3,4-dihydroxybenzoic acid, 3-hydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid or 4-acetamidobenzoic acid to the culture medium. After stabilizing the enzyme activity by adding 5 mM dithiothreitol and 20 mM Na2S2O3 to a cell-free extract, catechol at 50 mM was carboxylated in the presence of 3 M KHCO3 to 3,4-dihydroxybenzoic acid with a molar conversion ratio of 28% after 14 h at 30°C.

Keywords

CarboxylationCatechol3,4-Dihydroxybenzoate decarboxylaseEnterobacter cloacae

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Toyokazu Yoshida
    • 1
  • Yuki Inami
    • 1
  • Tsuyoshi Matsui
    • 1
  • Toru Nagasawa
    • 1
  1. 1.Department of Biomolecular ScienceGifu UniversityGifuJapan