Original Research Paper

Biotechnology Letters

, Volume 31, Issue 10, pp 1613-1616

Cationic polyacrylamides enhance rates of starch and cellulose saccharification

  • John T. ReyeAffiliated withInstitute of Paper Science and Technology, School of Chemical & Biomolecular Engineering, Georgia Institute of Technology
  • , Kendra MaxwellAffiliated withInstitute of Paper Science and Technology, School of Chemical & Biomolecular Engineering, Georgia Institute of Technology
  • , Swati RaoAffiliated withInstitute of Paper Science and Technology, School of Chemical & Biomolecular Engineering, Georgia Institute of Technology
  • , Jian LuAffiliated withInstitute of Paper Science and Technology, School of Chemical & Biomolecular Engineering, Georgia Institute of Technology
  • , Sujit BanerjeeAffiliated withInstitute of Paper Science and Technology, School of Chemical & Biomolecular Engineering, Georgia Institute of Technology Email author 

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Abstract

Adding a cationic polyacrylamide (c-PAM) to either the amylase mediated hydrolysis of corn starch or the hydrolysis of wood fiber by cellulase can enhance the initial hydrolysis rates, although a rate decrease can occur under some conditions. Several c-PAMs can serve as catalysts and the same c-PAM can improve the efficiency of both amylase and cellulase. The initial amylase rate approximately doubles; the analogous cellulase hydrolysis rate increases by about 40%. c-PAMs increase the binding of enzyme to substrate.

Keywords

Amylase Cellulose Polymer c-PAM Binding Rate