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Enhanced synthesis of l-threo-3,4-dihydroxyphenylserine by high-density whole-cell biocatalyst of recombinant l-threonine aldolase from Streptomyces avelmitilis

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Abstract

l-threo-3,4-Dihydroxyphenylserine (DOPS) is a chiral unnatural β-hydroxy amino acid used for the treatment of Parkinson disease. We developed a continuous bioconversion system for DOPS production that uses whole-cell biocatalyst of recombinant Escherichia coli expressing l-threonine aldolase (l-TA) genes cloned from Streptomyces avelmitilis MA-4680. Maximum conversion rates were observed at 2 M glycine, 145 mM 3,4-dihydroxybenzaldehyde, 0.75% Triton-X, 5 g E. coli cells/l, pH 6.5 and 10°C. In the optimized condition, overall productivity was 8 g/l, which represents 40 times the synthesis yield possible with no optimization of conditions.

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Acknowledgements

We thank Akita Tomi for technical assistance. This work was supported by the New Energy and Industrial Technology Development Organization (NEDO) in Japan and by Chonbuk National University.

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Correspondence to Sang-Ho Baik.

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Baik, SH., Yoshioka, H. Enhanced synthesis of l-threo-3,4-dihydroxyphenylserine by high-density whole-cell biocatalyst of recombinant l-threonine aldolase from Streptomyces avelmitilis . Biotechnol Lett 31, 443–448 (2009). https://doi.org/10.1007/s10529-008-9885-0

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  • DOI: https://doi.org/10.1007/s10529-008-9885-0

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