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Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration

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Abstract

We made a three-dimensional (3-D) nanofibrous fibroin scaffold (NFS) with high porosity (94%) and examined its feasibility in bone regeneration. Under scanning electron microscopy, MC3T3-E1 preosteoblasts on the scaffold showed more spread on the first day after seeding compared with a 2-D scaffold. MTT assay showed significantly increased proliferation in 3-D NFS compared with 2-D NFS 7 days after seeding (P < 0.05). Western immunoblotting for activated paxillin, FAK, AKT, C-Src, and ERK1/2 antibodies showed signals from the extracellular matrix were significantly increased in 3-D NFS. Newly developed 3-D electrospun NFS may be a good candidate for use in bone regeneration.

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Acknowledgements

This work was supported in part by grants from the SRC/ERC Program of MOST/ KOSEF (R11-2005-065) and the BioGreen 21 Program (Grant No. 20050401034685), Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul, 151921, Republic of Korea

    Chang Seok Ki & Young Hwan Park

  2. Intelligent Textile Research Center, Seoul National University, Seoul, 151-742, Republic of Korea

    Chang Seok Ki, Sook Young Park, Hyun Jeong Kim & Young Hwan Park

  3. Department of Dental Anesthesiology and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, 110-768, Republic of Korea

    Sook Young Park & Hyun Jeong Kim

  4. Department of Cell & Developmental Biology, School of Dentistry, Seoul National University, Seoul, 110-768, Republic of Korea

    Hong-Moon Jung & Kyung Mi Woo

  5. Cancer Research Institute, Department of Tumor Biology, College of Medicine, Seoul National University, Seoul, 110-799, Republic of Korea

    Jung Weon Lee

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  1. Chang Seok Ki
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  2. Sook Young Park
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  3. Hyun Jeong Kim
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  4. Hong-Moon Jung
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  5. Kyung Mi Woo
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  6. Jung Weon Lee
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  7. Young Hwan Park
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Corresponding authors

Correspondence to Hyun Jeong Kim or Young Hwan Park.

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Ki and Park equally contributed in this paper.

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Ki, C.S., Park, S.Y., Kim, H.J. et al. Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration. Biotechnol Lett 30, 405–410 (2008). https://doi.org/10.1007/s10529-007-9581-5

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  • DOI: https://doi.org/10.1007/s10529-007-9581-5

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