Skip to main content
SpringerLink
Log in

Mineralization of chlorpyrifos by co-culture of Serratia and Trichosporon spp.

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

A bacterial strain (Serratia sp.) that could transform chlorpyrifos to 3,5,6-trichloro-2-pyridinol (TCP) and a TCP-mineralizing fungal strain (Trichosporon sp.) were isolated from activated sludge by enrichment culture technique. The fungus could also degrade 50 mg chlorpyrifos l−1 within 7 days. Co-cultures completely mineralized 50 mg chlorpyrifos l−1 within 18 h at 30°C and pH 8 using a total inocula of 0.15 g biomass l−1.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Bazot S, Bois P, Joyeux C, Lebeau T (2007) Mineralization of diuron [3-(3,4-dichlorophenyl)-1, 1-dimethylurea] by co-immobilized Arthrobacter sp. and Delftia acidovorans. Biotechnol Lett 29:749–754

    Article  PubMed  CAS  Google Scholar 

  • Boszczyk MH, Chorazy M, Bieszkiewicz E, Kacieszczenko J (2002) Phenol utilisation by fungi isolated from activated sludge. Acta Microbiol Pol 51:183–191

    Google Scholar 

  • Bumpus JA, Kakar SN, Coleman RD (1993) Fungal degradation of organophosphorus insecticides. Appl Biochem Biotechnol 39–40:715–726

    Article  PubMed  Google Scholar 

  • Dejonghe W, Berteloot E, Goris J, Boon N, Crul K, Maertens S, Hofte M, De Vos P, Verstraete W, Top EM (2003) Synergistic degradation of linuron by a bacterial consortium and isolation of a single linuron-degrading Variovorax strain. Appl Environ Microbiol 69:1532–1541

    Article  PubMed  CAS  Google Scholar 

  • Environmental Protection Agency (1995) Review of chlorpyrifos poisoning data. US EPA 1–46

  • Feng Y, Racke KD, Bollag J (1997) Isolation and characterization of a chlorinated-pyridinol-degrading bacterium. Appl Environ Microbiol 63:4096–4098

    PubMed  CAS  Google Scholar 

  • Grant RJ, Betts WB (2004) Mineral and carbon usage of two synthetic pyrethroid degrading bacterial isolates. J Appl Microbiol 97:656–662

    Article  PubMed  CAS  Google Scholar 

  • Holt JG, Krieg NR, Sneath PH, Staley JT, Williams ST (1994) Bergey’s manual of determinative bacteriology, 9th edn. Williams and Wilkins, Baltimore, MD

    Google Scholar 

  • Li X, He J, Li S (2007) Isolation of a chlorpyrifos-degrading bacterium, Sphingomonas sp. strain Dsp-2, and cloning of the mpd gene. Res Microbiol 158:143–149

    Article  PubMed  CAS  Google Scholar 

  • Omar SA (1998) Availability of phosphorus and sulfur of insecticide origin by fungi. Biodegradation 9:327–336

    Article  PubMed  CAS  Google Scholar 

  • Pakala SB, Gorla P, Pinjari AB, Krovidi RK, Baru R, Yanamandra M, Merrick M, Siddavattam D (2007) Biodegradation of methyl parathion and p-nitrophenol: evidence for the presence of a p-nitrophenol 2-hydroxylase in a Gram-negative Serratia sp. strain DS001. Appl Microbiol Biotechnol 73:1452–1462

    Article  PubMed  CAS  Google Scholar 

  • Sietmann R, Gesell M, Hammer E, Schauer F (2006) Oxidative ring cleavage of low chlorinated biphenyl derivatives by fungi leads to the formation of chlorinated lactone derivatives. Chemosphere 64:672–685

    Article  PubMed  CAS  Google Scholar 

  • Singh BK, Walker A (2006) Microbial degradation of organophosphorus compounds. FEMS Microbiol Rev 30:428–471

    Article  PubMed  CAS  Google Scholar 

  • Singh BK, Walker A, Morgan JA, Wright DJ (2004) Biodegradation of chlorpyrifos by Enterobacter strain B-14 and its use in bioremediation of contaminated soils. Appl Environ Microbiol 70:4855–4863

    Article  PubMed  CAS  Google Scholar 

  • Soares A, Jonasson K, Terrazas E, Guieysse B, Mattiasson B (2005) The ability of white-rot fungi to degrade the endocrine-disrupting compound nonylphenol. Appl Microbiol Biotechnol 66:719–725

    Article  PubMed  CAS  Google Scholar 

  • Yang L, Zhao YH, Zhang BX, Yang CH, Zhang X (2005) Isolation and characterization of a chlorpyrifos and 3,5,6-trichloro-2-pyridinol degrading bacterium. FEMS Microbiol Lett 251:67–73

    Article  PubMed  CAS  Google Scholar 

  • Yang C, Liu N, Guo X, Qiao C (2006) Cloning of mpd gene from a chlorpyrifos-degrading bacterium and use of this strain in bioremediation of contaminated soil. FEMS Microbiol Lett 265:118–125

    Article  PubMed  CAS  Google Scholar 

  • Yu YL, Fang H, Wang X, Wu XM, Shan M, Yu JQ (2006) Characterization of a fungal strain capable of degrading chlorpyrifos and its use in detoxification of the insecticide on vegetables. Biodegradation 17:487–494

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study was sponsored by the Hi-tech Research and Development Program of China (No.2006AA10Z401). We are indebted to Min Xia, Xinxin Wang, and Xiaoman Zhang (Beijing Center for Physical and Chemical Analysis, China) for providing facilities and helping with GC and HPLC analysis.

Author information

Authors and Affiliations

  1. State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, Shandong, 271018, P.R. China

    Gangming Xu, Yingying Li, Wei Zheng, Xiang Peng & Wen Li

  2. College of Life Sciences, Shandong Agricultural University, Tai’an, Shandong, 271018, P.R. China

    Gangming Xu, Yingying Li, Wei Zheng, Xiang Peng & Wen Li

  3. Graduate School, Chinese Academy of Agricultural Sciences, Beijing, 100081, P.R. China

    Yanchun Yan

Authors
  1. Gangming Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yingying Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wei Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiang Peng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wen Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yanchun Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yanchun Yan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xu, G., Li, Y., Zheng, W. et al. Mineralization of chlorpyrifos by co-culture of Serratia and Trichosporon spp.. Biotechnol Lett 29, 1469–1473 (2007). https://doi.org/10.1007/s10529-007-9444-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-007-9444-0

Keywords

Search

Navigation